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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

Full text
Author(s):
Souza, Sivoney F. ; Kogikoski, Jr., Sergio ; Silva, Emerson R. ; Alves, Wendel A.
Total Authors: 4
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 28, n. 9, p. 1619-1629, SEP 2017.
Web of Science Citations: 4
Abstract

Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions. (AU)

FAPESP's process: 13/12674-6 - Structural investigations on model systems based on amphiphilic peptides
Grantee:Emerson Rodrigo da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/24018-1 - Polymer-Peptide Conjugates for hydrogel formulation and its usage in detection
Grantee:Wendel Andrade Alves
Support type: Regular Research Grants
FAPESP's process: 08/57805-2 - Institute of Bioanalytics
Grantee:Lauro Tatsuo Kubota
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/01933-8 - Biomimetic electrospun matrixes: possible application in NO detection
Grantee:Sergio Kogikoski Junior
Support type: Scholarships in Brazil - Doctorate (Direct)