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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anatomical Organization of Urocortin 3-Synthesizing Neurons and Immunoreactive Terminals in the Central Nervous System of Non-Human Primates [Sapajus spp.]

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Battagello, Daniella S. ; Diniz, Giovanne B. ; Candido, Paulo L. ; da Silva, Joelcimar M. ; de Oliveira, Amanda R. ; da Silva, Kelly R. Torres ; Lotfi, Claudimara F. P. ; de Oliveira, Jose A. ; Sita, Luciane V. ; Casatti, Claudio A. ; Lovejoy, David A. ; Bittencourt, Jackson C.
Total Authors: 12
Document type: Journal article
Source: FRONTIERS IN NEUROANATOMY; v. 11, JUL 24 2017.
Web of Science Citations: 1
Abstract

Urocortin 3 (UCN3) is a neuropeptide member of the corticotropin-releasing factor (CRF) peptide family that acts as a selective endogenous ligand for the CRF, subtype 2 (CRF2) receptor. Immunohistochemistry and in situ hybridization data from rodents revealed UCN3-containing neurons in discrete regions of the central nervous system (CNS), such as the medial preoptic nucleus, the rostral perifornical area (PFA), the medial nucleus of the amygdala and the superior paraolivary nucleus. UCN3-immunoreactive (UCN3-ir) terminals are distributed throughout regions that mostly overlap with regions of CRF2 messenger RNA (mRNA) expression. Currently, no similar mapping exists for non-human primates. To better understand the role of this neuropeptide, we aimed to study the UCN3 distribution in the brains of New World monkeys of the Sapajus genus. To this end, we analyzed the gene and peptide sequences in these animals and performed immunohistochemistry and in situ hybridization to identify UCN3 synthesis sites and to determine the distribution of UCN3-ir terminals. The sequencing of the Sapajus spp. UCN3-coding gene revealed 88% and 65% identity to the human and rat counterparts, respectively. Additionally, using a probe generated from monkey cDNA and an antiserum raised against human UCN3, we found that labeled cells are mainly located in the hypothalamic and limbic regions. UCN3-ir axons and terminals are primarily distributed in the ventromedial hypothalamic nucleus (VMH) and the lateral septal nucleus (LS). Our results demonstrate that UCN3-producing neurons in the CNS of monkeys are phylogenetically conserved compared to those of the rodent brain, that the distribution of fibers agrees with the distribution of CRF2 in other primates and that there is anatomical evidence for the participation of UCN3 in neuroendocrine control in primates. (AU)

FAPESP's process: 11/09816-8 - Anatomical substrate for the neuroendocrine role of the incerto-hypothalamic area
Grantee:Luciane Valéria Sita
Support type: Regular Research Grants
FAPESP's process: 04/13849-5 - Peptidergic pathways involved in the organization of feeding behavior
Grantee:Jackson Cioni Bittencourt
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/06769-9 - Distribution of urocortin 3 (UCN 3) in the central nervous system of primate (Cebus apella)
Grantee:Daniella Sabino Battagello
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/03067-6 - Expression of teneurins (TEN) and teneurins C-terminal associated peptides (TCAP) in the central nervous system of non-human primates (Cebus apella)
Grantee:Cláudio Aparecido Casatti
Support type: Regular Research Grants
FAPESP's process: 12/08833-9 - Expression of teneurins and teneurins C-terminal associated peptides (TCAPs) in the central nervous system of non-human primates (Cebus apella)
Grantee:Kelly Regina Torres da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/02748-0 - Study of the anatomical basis for a volume transmission mechanism on the Melanin Concentrating Hormone [MCH] peptidergic system
Grantee:Giovanne Baroni Diniz
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/02224-1 - The existence of neurogenesis during the lactation period
Grantee:Jackson Cioni Bittencourt
Support type: Research Projects - Thematic Grants