| Full text | |
| Author(s): |
Ferraz, Emanuela Prado
;
Freitas, Gileade Pereira
;
Crovace, Murilo Camuri
;
Peitl, Oscar
;
Zanotto, Edgar Dutra
;
de Oliveira, Paulo Tambasco
;
Beloti, Marcio Mateus
;
Rosa, Adalberto Luiz
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Biomedical Materials; v. 12, n. 4 AUG 25 2017. |
| Web of Science Citations: | 4 |
| Abstract | |
We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded with MSCs. UMR-106 proliferation was similar for both materials, while alkaline phosphatase (ALP) activity and mineralization were higher for BioS-2P. Bone sialoprotein (BSP), RUNX2 and osteopontin (OPN) gene expression and BSP, OPN, ALP and RUNX2 protein expression were higher on BioS-2P. For MSCs, ALP activity was higher on Bioglass 45S5 than on BioS-2P and was lower on polystyrene. All genes were highly expressed on bioactive glasses compared to polystyrene. BioS-2P scaffolds promoted in vivo bone formation without differences in the morphometric parameters at 4, 8 and 12 weeks. After 8 weeks, the combination of BioS-2P with MSCs did not increase the quantity of new bone compared to the BioS-2P alone. To stimulate osteoblast activity, drive MSC differentiation and promote bone formation, BioS-2P is a good choice as a scaffold for bone tissue engineering. (AU) | |
| FAPESP's process: | 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass |
| Grantee: | Edgar Dutra Zanotto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 12/23879-5 - Tissue engineering: effect of the combination of cells and Bio-sca on bone defect repair |
| Grantee: | Adalberto Luiz Rosa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/23525-9 - TISSUE ENGINEERING: EFFECT OF THE COMBINATION OF CELLS AND BIO-SCA ON BONE DEFECT REPAIR |
| Grantee: | Emanuela Prado Ferraz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |