Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

Full text
Author(s):
Pereira da Silva, Victor Hugo ; de Luna Antonio, Raquel ; Pompeia, Sabine ; Ribeiro, Daniel Araki
Total Authors: 4
Document type: Journal article
Source: ACTA CYTOLOGICA; v. 59, n. 6, p. 474-478, 2015.
Web of Science Citations: 8
Abstract

Objective: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. Study Design: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. Results: Smokers presented more (p < 0.05) micronucleated oral mucosa cells than non-smokers. Tobacco smoke was not able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis. Conclusion: In summary, these data indicate that the cigarette is able to induce micronuclei in oral mucosa cells, so the micronucleus test is a suitable method for predicting oral cancer risk. (C) 2016 S. Karger AG, Basel (AU)

FAPESP's process: 13/10985-4 - Cognitive profile in tobacco smokers: relationship with respiratory parameters and oxidative stress
Grantee:Sabine Pompéia
Support type: Regular Research Grants
FAPESP's process: 12/20664-8 - Cognitive profile in tobacco smokers: relationship with respiratory parameters and oxidative stress
Grantee:Raquel de Luna Antonio
Support type: Scholarships in Brazil - Doctorate