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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration

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Beltrame, Flavia L. ; Sasso-Cerri, Estela
Total Authors: 2
Document type: Journal article
Source: ASIAN JOURNAL OF ANDROLOGY; v. 19, n. 5, p. 567-572, SEP-OCT 2017.
Web of Science Citations: 3

The H-2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B-12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with vitamin B-12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether vitamin B-12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg(-1) b.w. of cimetidine, cimetidine-B-12 group received cimetidine and 3 mu g of vitamin B-12-hydroxocobalamin, B-12 group received only 3 mu g of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (A; In/B) and spermatocytes. TUNEL method and PCNA immunofluorescence were performed. Cimetidine caused a significant reduction in sperm concentration. TUNEL-positive spermatogonia and spermatocytes were correlated to a significant reduction in the number of these cells. In cimetidine-B-12 group, sperm concentration was higher than cimetidine group and a significant increase in the number of spermatogonia (stages II-VI) was correlated to a high incidence of PCNA-immunolabeled spermatogonia and spermatocytes. The results show that the supplementation of rats with vitamin B-12 during cimetidine treatment increases sperm concentration and exerts a potential effect in the recovery of spermatogonia and spermatocytes. (AU)

FAPESP's process: 12/23845-3 - Evaluation of Vitamin B12 effect on the mitotic and meiotic phases of spermatogenesis and in the sperm DNA of cimetidine-treated rats
Grantee:Estela Sasso Cerri
Support type: Regular Research Grants
FAPESP's process: 13/25322-0 - Effect of cimetidine on the epididymal histoarchitecture and evaluation of sperm DNA of rats treated with cimetidine and supplemented with B12
Grantee:Flávia Luciana Beltrame
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/02409-5 - Histopathological evaluation of interstitial tissue in cimetidine-treated rats and role of vitamin B12 on the spermatogenic repair
Grantee:Flávia Luciana Beltrame
Support type: Scholarships in Brazil - Doctorate