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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enzyme Prodrug Therapy Engineered into Electrospun Fibers with Embedded Liposomes for Controlled, Localized Synthesis of Therapeutics

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Chandrawati, Rona ; Olesen, Morten T. J. ; Marini, Thatiane C. C. ; Bisra, Gurpal ; Guex, Anne Geraldine ; de Oliveira, Marcelo G. ; Zelikin, Alexander N. ; Stevens, Molly M.
Total Authors: 8
Document type: Journal article
Source: ADVANCED HEALTHCARE MATERIALS; v. 6, n. 17 SEP 6 2017.
Web of Science Citations: 11

Enzyme prodrug therapy (EPT) enables localized conversion of inert prodrugs to active drugs by enzymes. Performance of EPT necessitates that the enzyme remains active throughout the time frame of the envisioned therapeutic application. beta-glucuronidase is an enzyme with historically validated performance in EPT, however it retains its activity in biomaterials for an insufficiently long period of time, typically not exceeding 7 d. Herein, the encapsulation of beta-glucuronidase in liposomal subcompartments within poly(vinyl alcohol) electrospun fibers is reported, leading to the assembly of biocatalytically active materials with activity of the enzyme sustained over at least seven weeks. It is further shown that liposomes provide the highly beneficial stabilization of the enzyme when incubated in cell culture media. The assembled biocatalytic materials successfully produce antiproliferative drugs (SN-38) using externally administered prodrugs (SN-38-glucuronide) and effectively suppress cell proliferation, with envisioned utility in the design of cardiovascular grafts. (AU)

FAPESP's process: 14/03266-4 - PVA-F-127 thermosensitive nanocarriers for the photochemical nitric oxide release
Grantee:Thatiane Cristine Camargo Marini
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/13290-2 - 27th European Conference on Biomaterials
Grantee:Marcelo Ganzarolli de Oliveira
Support type: Research Grants - Meeting - Abroad