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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Schistosoma mansoni genome encodes thousands of long non-coding RNAs predicted to be functional at different parasite life-cycle stages

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Author(s):
Vasconcelos, Elton J. R. ; dasilva, Lucas F. ; Pires, David S. ; Lavezzo, Guilherme M. ; Pereira, Adriana S. A. ; Amaral, Murilo S. ; Verjovski-Almeida, Sergio
Total Authors: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, SEP 5 2017.
Web of Science Citations: 9
Abstract

Next Generation Sequencing (NGS) strategies, like RNA-Seq, have revealed the transcription of a wide variety of long non-coding RNAs (lncRNAs) in the genomes of several organisms. In the present work we assessed the lncRNAs complement of Schistosoma mansoni, the blood fluke that causes schistosomiasis, ranked among the most prevalent parasitic diseases worldwide. We focused on the long intergenic/intervening ncRNAs (lincRNAs), hidden within the large amount of information obtained through RNA-Seq in S. mansoni (88 libraries). Our computational pipeline identified 7029 canonically-spliced putative lincRNA genes on 2596 genomic loci (at an average 2.7 isoforms per lincRNA locus), as well as 402 spliced lncRNAs that are antisense to protein-coding (PC) genes. Hundreds of lincRNAs showed traits for being functional, such as the presence of epigenetic marks at their transcription start sites, evolutionary conservation among other schistosome species and differential expression across five different life-cycle stages of the parasite. Real-time qPCR has confirmed the differential life-cycle stage expression of a set of selected lincRNAs. We have built PC gene and lincRNA co-expression networks, unraveling key biological processes where lincRNAs might be involved during parasite development. This is the first report of a large-scale identification and structural annotation of lncRNAs in the S. mansoni genome. (AU)

FAPESP's process: 16/10046-6 - Effect of GSK343, an inhibitor of the histone methyltransferase EZH2, on the parasite Schistosoma mansoni
Grantee:Adriana Silva Andrade Pereira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/24560-8 - Identification and characterization of regulatory Long Non-coding RNAs on the Schistosoma mansoni genome through NGS strategies and systems approach
Grantee:Elton José Rosas de Vasconcelos
Support type: Scholarships in Brazil - Post-Doctorate