Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ArtinM Mediates Murine T Cell Activation and Induces Cell Death in Jurkat Human Leukemic T Cells

Full text
Author(s):
da Silva, Thiago Aparecido ; Martins Oliveira-Brito, Patricia Kellen ; Goncalves, Thiago Eleuterio ; Vendruscolo, Patricia Edivania ; Roque-Barreira, Maria Cristina
Total Authors: 5
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 18, n. 7 JUL 2017.
Web of Science Citations: 5
Abstract

The recognition of cell surface glycans by lectins may be critical for the innate and adaptive immune responses. ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing TLR2 N-glycans and induces Th1 immunity. We recently demonstrated that ArtinM stimulated CD4(+) T cells to produce proinflammatory cytokines. Here, we further studied the effects of ArtinM on adaptive immune cells. We showed that ArtinM activates murine CD4(+) and CD8(+) T cells, augmenting their positivity for CD25, CD69, and CD95 and showed higher interleukin (IL)-2 and interferon (IFN)-gamma production. The CD4(+) T cells exhibited increased T-bet expression in response to ArtinM, and IL-2 production by CD4(+) and CD8(+) T cells depended on the recognition of CD3 epsilon gamma-chain glycans by ArtinM. The ArtinM effect on aberrantly-glycosylated neoplastic lymphocytes was studied in Jurkat T cells, in which ArtinM induced IL-2, IFN-gamma, and IL-1 beta production, but decreased cell viability and growth. A higher frequency of AnnexinV -and propidium iodide-stained cells demonstrated the induction of Jurkat T cells apoptosis by ArtinM, and this apoptotic response was reduced by caspases and protein tyrosine kinase inhibitors. The ArtinM effects on murine T cells corroborated with the immunomodulatory property of lectin, whereas the promotion of Jurkat T cells apoptosis may reflect a potential applicability of ArtinM in novel strategies for treating lymphocytic leukemia. (AU)

FAPESP's process: 14/16003-1 - Effect of adoptive transfer of spleen cells or CD4+ T cells stimulated with ArtinM during infection with Paracoccidioides brasiliensis
Grantee:Thiago Eleutério Gonçalves
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/23112-7 - The immunomodulatory activity of ArtinM on the course of Cryptococcus gattii infection
Grantee:Patrícia Kellen Martins Oliveira Brito
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/10446-4 - Mechanisms of immunomodulation by ArtinM: basis for the development of new anti-fungal therapy
Grantee:Maria Cristina Roque Antunes Barreira
Support Opportunities: Regular Research Grants
FAPESP's process: 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis
Grantee:Thiago Aparecido da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 06/60642-2 - Lectins: biological effects and pharmaceutical applications
Grantee:Maria Cristina Roque Antunes Barreira
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/09611-0 - Effect of lectin ArtinM on murine CD4+ T and CD8+ T cells
Grantee:Thiago Aparecido da Silva
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/04088-0 - Lectin from pathogens
Grantee:Maria Cristina Roque Antunes Barreira
Support Opportunities: Research Projects - Thematic Grants