Liver steatosis in hypothalamic obese rats improves after duodeno-jejunal bypass by reduction in de novo lipogenesis pathway

Author(s)

Soares, Gabriela Moreira ^[1] ; Cantelli, Kathia Regina ^[1] ; Balbo, Sandra Lucinei ^[1] ; Ribeiro, Rosane Aparecida ^[2] ; Paiva Alegre-Maller, Ana Claudia ^[1] ; Barbosa-Sarnpaio, Helena Cristina ^[3] ; Boschero, Antonio Carlos ^[3] ; Faria Araujo, Allan Cezar ^[4] ; Bonfleur, Maria Lucia ^[1]

Affiliation

^[1] Univ Estadual Oeste Parana UNIOESTE, Ctr Ciencias Biol & Saude, Lab Fisiol Endocrina & Metab LAFEM, Cascavel, PR - Brazil

^[2] Univ Fed Rio de Janeiro UFRJ, Campus UFRJ Macae, Macae, RJ - Brazil

^[3] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Estrutural & Func, Lab Pancreas Endocrino & Metab, Campinas, SP - Brazil

^[4] Univ Estadual Oeste Parana, Ctr Ciencias Med & Farmaceut, Cascavel, PR - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 188, p. 68-75, NOV 1 2017.
• Web of Science Citations: 1

Abstract

Aims: Hypothalamic obesity is a severe condition without any effective therapy. Bariatric operations appear as an alternative treatment, but the effects of this procedure are controversial. We, herein, investigated the effects of duodeno-jejunal bypass (DJB) surgery upon the lipid profile and expression of genes and proteins, involved in the regulation of hepatic lipid metabolism, in hypothalamic obese (IIyO) rats. Methods: During the first 5 days of life, male newborn Wistar rats received subcutaneous injections of monosodium glutamate {[}4 g/kg body weight, HyO group] or saline (control, CTL group). At 90 days of life, HyO rats were randomly submitted to DJB (HyO DJB) or Sham-operations (HyO Sham group). Six months after DJB, adiposity, hepatic steatosis and lipid metabolism were verified. Key findings: HyO Sham rats were obese, hyperinsulinemic, insulin resistant and dyslipidemic. These rats had higher liver contents of trygliceride (TG) and presented disorganization of the hepatocyte structures, in association with higher hepatic contents of acetyl-CoA carboxylase (ACC), fatty acid synthase (FASN), and stearoyl-CoA desaturase-1 mRNAs and protein. DJB surgery normalized insulinemia, insulin resistance, and dyslipidemia in HyO rats. TG content in the liver and the hepatic microscopic structures were also normalized in HyO DJB rats, while the expressions of ACC and FASN proteins were decreased in the liver of these rodents. Significance: The DJB-induced amelioration in hepatic steatosis manifested as a late effect in HyO rats, and was partly associated with a downregulation in hepatic de novo lipogenesis processes, indicating that DJB protects against liver steatosis in hypothalamic obesity. (AU)

• FAPESP's process: 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass
• Grantee: Antonio Carlos Boschiero
• Support Opportunities: Research Projects - Thematic Grants