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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficient Non-Invasive Plasmid-DNA Administration into Tibialis Cranialis Muscle of ``Little{''} Mice

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Author(s):
Cecchi, C. R. [1] ; Higuti, E. [1] ; Lima, E. R. [1] ; Vieira, D. P. [1] ; Squair, P. L. [2] ; Peroni, C. N. [1] ; Bartolini, P. [1]
Total Authors: 7
Affiliation:
[1] Inst Pesquisas Energet & Nucl IPEN CNEN, Biotechnol Ctr, Cidade Univ, Sao Paulo, SP - Brazil
[2] Inst Pesquisas Energet & Nucl IPEN CNEN, Radiopharm Ctr, Cidade Univ, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CURRENT MOLECULAR MEDICINE; v. 17, n. 3, p. 230-235, 2017.
Web of Science Citations: 0
Abstract

Background: An alternative treatment for growth hormone deficiency based on hGH-DNA administration, followed by electro gene transfer, was investigated by injecting the plasmid into surgically exposed or non-exposed quadriceps or tibialis muscle of immunodeficient ``little{''} mice. Methods: An optimization of electrotransfer conditions via a new combination of high/low voltage pulses is presented. After 3 days, serum hGH was determined and in a 28-day assay, the relative growth parameters were compared. Results: Both groups exhibited similar results: 5.0 +/- 2.2 (SD) and 3.5 +/- 0.9 ng hGH/ml (P>0.05; n=7) for the exposed quadriceps and non-exposed tibialis treatments, respectively. The final body weight increases were 16.1% for the quadriceps and 18.9% for the tibialis group. The tail and nose-to-tail length increases were 4.5% and 7.1% for the quadriceps and 4.8 and 4.6% for the tibialis group. The right and left femur length increases, obtained from radiographic measurements, were 16.9% and 12.7% for the quadriceps and 19.4% and 12.3% for the tibialis, respectively. A non-significant difference between exposed quadriceps and non-exposed tibialis treatments (P=0.48) was confirmed via a completely integrated statistical analysis. Circulating mIGF-1 levels were 126 +/- 47, 106 +/- 93 (P>0.05) and 38 +/- 15 ng/ml for the quadriceps, tibialis and saline treatments, respectively. Conclusion: These results show that hGH-DNA administration into non-exposed tibialis muscle followed by the new HV/LV electrotransfer protocol was an equally efficient, less traumatic treatment, much more suitable for pre-clinical testing than administration into exposed quadriceps. (AU)

FAPESP's process: 14/19757-7 - Growth hormone gene therapy: plasmid DNA injection and microencapsulated cells implantation in dwarf mice
Grantee:Cibele Nunes Peroni
Support type: Regular Research Grants
FAPESP's process: 14/04277-0 - Growth Hormone Gene therapy: homologous treatment based on plasmid DNA administration and on microencapsulated cells implanted in immunocompetent dwarf mice (lit/lit)
Grantee:Cláudia Regina Cecchi
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 14/18242-3 - Gene therapy for growth hormone deficiency mediated by minicircle DNA electrotransfer in dwarf mice
Grantee:Eliza Higuti Sousa
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/03747-0 - Growth Hormone Gene Therapy: homologous treatment with plasmid DNA and with implant of microencapsulated cells in immunocompetent dwarf mice (lit/lit)
Grantee:Cláudia Regina Cecchi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/07380-6 - Optimization of in vivo growth hormone gene transfer parameters for phenotypic correction of dwarf mice
Grantee:Eliana Rosa Lima Filha
Support type: Scholarships in Brazil - Master