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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spectroscopic and calorimetric assays reveal dependence on dCTP and two metals (Zn2+ + Mg2+) for enzymatic activity of Schistosoma mansoni deoxycytidylate (dCMP) deaminase

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Author(s):
Scortecci, Jessica Fernandes [1] ; Balasco Serrao, Vitor Hugo [1, 2, 3] ; Cheleski, Juliana [1] ; Torini, Juliana Roberta [1] ; Romanello, Larissa [1] ; DeMarco, Ricardo [1] ; Pereira, Humberto D'Muniz [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[2] Harvard Med Sch, Dept Neurobiol, Boston, MA 02115 - USA
[3] 220 Longwood Ave, Boston, MA - USA
Total Affiliations: 3
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1865, n. 11, A, p. 1326-1335, NOV 2017.
Web of Science Citations: 1
Abstract

The parasite Schistosoma mansoni possess all pathways for pyrimidine biosynthesis, whereby deaminases play an essential role in the thymidylate cycle, a crucial step to controlling the ratio between cytidine and uridine nucleotides. In this study, we heterologously expressed and purified the deoxycytidylate (dCMP) deaminase from S. mansoni to obtain structural, biochemical and kinetic information. Small-angle X-ray scattering of this enzyme showed that it is organized as a hexamer in solution. Isothermal titration calorimetry was used to determine the kinetic constants for dCMP-dUMP conversion and the role of dCTP and dTTP in enzymatic regulation. We evaluated the metals involved in activating the enzyme and show for the first time the dependence of correct folding on the interaction of two metals. This study provides information that may be useful for understanding the regulatory mechanisms involved in the metabolic pathways of S. mansoni. Thus, improving our understanding of the function of these essential pathways for parasite metabolism and showing for the first time the hitherto unknown deaminase function in this parasite. (AU)

FAPESP's process: 12/05532-8 - Structural and kinetic studies of adenosine kinase, hypoxanthine-guanine phosphoribosyltransferase, Adenilsuccinate synthetase and Adenilsuccinato lyase enzymes from Schistosoma mansoni
Grantee:Larissa Romanello
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/14223-9 - Structural and kinetical studies of the enzymes involved in the purine metabolism in Schistosoma mansoni
Grantee:Humberto D'Muniz Pereira
Support type: Regular Research Grants
FAPESP's process: 12/23730-1 - Characterization of macromolecular interactions of proteins involved in the selenocysteine synthesis from Escherichia coli
Grantee:Vitor Hugo Balasco Serrão
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/15777-8 - Kinetic and structural studies of the Deoxycytidylate deaminase (DEOC) from Schistosoma mansoni
Grantee:Jessica Fernandes Scortecci
Support type: Scholarships in Brazil - Scientific Initiation