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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of SOS-induced levels of imuABC on spontaneous and damage-induced mutagenesis in Caulobacter crescentus

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Alves, Ingrid R. [1] ; Lima-Noronha, Marco A. [1] ; Silva, Larissa G. [1] ; Fernandez-Silva, Frank S. [1] ; Freitas, Aline Luiza D. [1] ; Marques, Marilis V. [1] ; Galhardo, Rodrigo S. [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: DNA Repair; v. 59, p. 20-26, NOV 2017.
Web of Science Citations: 3

imuABC (brtuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents. Nevertheless; the presence of the operator-constitutive mutation rescues mutagenesis in a recA background, indicating that imuABC are the only genes required at SOS-induced levels for translesion synthesis (TLS) in C. crescentus. Furthermore, these data also show that TLS mediated by ImuABC does not require RecA, unlike umuDC-dependent mutagenesis in E. coli. (AU)

FAPESP's process: 14/15982-6 - Consequences of repair deficiencies in damaged genome
Grantee:Carlos Frederico Martins Menck
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/04046-8 - Regulatory networks of bacterial stress response
Grantee:Marilis Do Valle Marques
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/23133-6 - Molecular studies of operon imuAB dnaE2 in Caulobacter crescentus
Grantee:Marco Antonio de Lima Noronha
Support Opportunities: Scholarships in Brazil - Scientific Initiation