| Full text | |
| Author(s): |
Alves, Ingrid R.
[1]
;
Lima-Noronha, Marco A.
[1]
;
Silva, Larissa G.
[1]
;
Fernandez-Silva, Frank S.
[1]
;
Freitas, Aline Luiza D.
[1]
;
Marques, Marilis V.
[1]
;
Galhardo, Rodrigo S.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | DNA Repair; v. 59, p. 20-26, NOV 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
imuABC (brtuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents. Nevertheless; the presence of the operator-constitutive mutation rescues mutagenesis in a recA background, indicating that imuABC are the only genes required at SOS-induced levels for translesion synthesis (TLS) in C. crescentus. Furthermore, these data also show that TLS mediated by ImuABC does not require RecA, unlike umuDC-dependent mutagenesis in E. coli. (AU) | |
| FAPESP's process: | 14/15982-6 - Consequences of repair deficiencies in damaged genome |
| Grantee: | Carlos Frederico Martins Menck |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/04046-8 - Regulatory networks of bacterial stress response |
| Grantee: | Marilis Do Valle Marques |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/23133-6 - Molecular studies of operon imuAB dnaE2 in Caulobacter crescentus |
| Grantee: | Marco Antonio de Lima Noronha |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |