Is DNA ploidy related to smoking? - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Is DNA ploidy related to smoking?

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Author(s):
Lima, Celina Faig [1, 2] ; Oliveira Alves, Monica Ghislaine [1, 2] ; do Carmo Carvalho, Bruna Fernandes [1] ; de Lima, Thaynara Alves [1] ; Coutinho-Camillo, Claudia Malheiros [3] ; Soares, Fernando Augusto [3] ; Scholz, Jaqueline [4] ; Almeida, Janete Dias [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
[2] Univ Braz Cubas, Sch Dent, Mogi Das Cruzes - Brazil
[3] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Univ Hosp, Heart Inst Sao Paulo, Med Sch, Outpatient Program Treatment Smoking, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 46, n. 10, p. 961-966, NOV 2017.
Web of Science Citations: 0
Abstract

BackgroundIn the oral cavity, genomic instability is caused by long-term exposure to carcinogens. The aim of this study was to evaluate the relationship between smoking and DNA ploidy. MethodsCytological material was obtained from patients participating in the Outpatient Smoking Treatment Program of the Heart Institute (INCOR-HCFMUSP), and of the Discipline of Oral Medicine (ICT-UNESP). The inclusion criteria for all groups were the absence of a history of malignant tumors, absence of clinical signs of changes in the selected area, and alcohol consumption of less than 3 units per week. Group 1:30 smokers before smoking cessation treatment; Group 2:30 non-smokers; Group 3:30 ex-smokers abstinent for at least 1 year. Cytological smears were collected from the floor of the mouth and border of the tongue and stained by Feulgen. Aneuploidy was evaluated using the ACIS (R) III system. ResultsThe Kruskal-Wallis test showed no statistically significant difference (P = .4383) between the groups studied. No association between tobacco consumption and aneuploidy was observed in group 1 (P = 1) or group 2 (P = .68; Fisher's exact test). ConclusionSmoking was not associated with changes in DNA content or the incidence of aneuploidy in normal oral mucosa. (AU)

FAPESP's process: 12/05371-4 - Cytogenetic evaluation of smoking damage in oral mucosa
Grantee:Janete Dias Almeida
Support Opportunities: Regular Research Grants
FAPESP's process: 13/09910-0 - Effect of chronic smoking and abstinence on aneuploidy in oral mucosa cells
Grantee:Bruna Fernandes do Carmo Carvalho
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/02168-6 - EFFECT OF CHRONIC SMOKING ON DNA GENE REPAIR METHYLATION IN ORAL MUCOSA.
Grantee:Celina Faig Lima Carta
Support Opportunities: Scholarships in Brazil - Post-Doctoral