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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early Postnatal Cardiomyocyte Proliferation Requires High Oxidative Energy Metabolism

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Author(s):
Teofilo Saturi de Carvalho, Ana Elisa [1] ; Bassaneze, Vinicius [1] ; Forni, Maria Fernanda [2] ; Keusseyan, Aline Alfonso [1] ; Kowaltowski, Alicia Juliana [2] ; Krieger, Jose Eduardo [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Med Sch, Lab Genet & Mol Cardiol LIM 13, Heart Inst, InCor HCFMUSP, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, NOV 13 2017.
Web of Science Citations: 6
Abstract

Cardiac energy metabolism must cope with early postnatal changes in tissue oxygen tensions, hemodynamics, and cell proliferation to sustain development. Here, we tested the hypothesis that proliferating neonatal cardiomyocytes are dependent on high oxidative energy metabolism. We show that energy-related gene expression does not correlate with functional oxidative measurements in the developing heart. Gene expression analysis suggests a gradual overall upregulation of oxidative-related genes and pathways, whereas functional assessment in both cardiac tissue and cultured cardiomyocytes indicated that oxidative metabolism decreases between the first and seventh days after birth. Cardiomyocyte extracellular flux analysis indicated that the decrease in oxidative metabolism between the first and seventh days after birth was mostly related to lower rates of ATP-linked mitochondrial respiration, suggesting that overall energetic demands decrease during this period. In parallel, the proliferation rate was higher for early cardiomyocytes. Furthermore, in vitro nonlethal chemical inhibition of mitochondrial respiration reduced the proliferative capacity of early cardiomyocytes, indicating a high energy demand to sustain cardiomyocyte proliferation. Altogether, we provide evidence that early postnatal cardiomyocyte proliferative capacity correlates with high oxidative energy metabolism. The energy requirement decreases as the proliferation ceases in the following days, and both oxidative-dependent metabolism and anaerobic glycolysis subside. (AU)

FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 13/26440-7 - Identification of surface markers in cardiac precursors of rats
Grantee:Aline Alfonso Keusseyan
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/10109-7 - Mitochondrial energy metabolism and cardiomyogenesis for cardiac regeneration
Grantee:Ana Elisa Teófilo Saturi de Carvalho
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/17368-0 - Cardiovascular genomics: mechanisms & novel therapeutics - CVGen mech2ther
Grantee:José Eduardo Krieger
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/04871-6 - Effects of caloric restriction on mitochondrial morphology, dynamics, bioenergetics and redox state
Grantee:Maria Fernanda Pereira de Araújo Demonte Forni
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/19678-1 - Cardiac progenitor cells identification and isolation using aptamers
Grantee:Vinícius Bassaneze
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/25776-7 - Mitochondrial partitioning in mammalian cells
Grantee:Maria Fernanda Pereira de Araújo Demonte Forni
Support type: Scholarships in Brazil - Post-Doctorate