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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy

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Author(s):
Trigo Gutierrez, Jeffersson Krishan [1] ; Zanatta, Gabriela Cristina [1] ; Mira Ortega, Ana Laura [1] ; Cuba Balastegui, Maria Isabella [1] ; Sanita, Paula Volpato [1] ; Pavarina, Ana Claudia [1] ; Barbugli, Paula Aboud [1] ; de Oliveira Mima, Ewerton Garcia [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 12, n. 11 NOV 6 2017.
Web of Science Citations: 11
Abstract

Curcumin (CUR) has been used as photosensitizer in antimicrobial Photodynamic Therapy (aPDT). However its poor water solubility, instability, and scarce bioavalibility hinder its in vivo application. The aim of this study was to synthesize curcumin in polymeric nanoparticles (NP) and to evaluate their antimicrobial photodynamic effect and cytoxicity. CUR in anionic and cationic NP was synthesized using polylactic acid and dextran sulfate by the nanoprecipitation method. For cationic NP, cetyltrimethylammonium bromide was added. CUR-NP were characterized by physicochemical properties, photodegradation, encapsulation efficiency and release of curcumin from nanoparticles. CUR-NP was compared with free CUR in 10% dimethyl sulfoxide (DMSO) as a photosensitizer for aPDT against planktonic and biofilms (mono-, dual-and triple-species) cultures of Streptococcus mutans, Candida albicans and Methicillin-Resistant Staphylococcus aureus. The cytotoxicity effect of formulations was evaluated on keratinocytes. Data were analysed by parametric (ANOVA) and non-parametric (Kruskal-Wallis) tests (alpha = 0.05). CUR-NP showed alteration in the physicochemical properties along time, photodegradation similar to free curcumin, encapsulation efficiency up to 67%, and 96% of release after 48h. After aPDT planktonic cultures showed reductions from 0.78 loglo to complete eradication, while biofilms showed no antimicrobial effect or reductions up to 4.44 loglo. Anionic CUR-NP showed reduced photoinactivation of biofilms. Cationic CUR-NP showed microbicidal effect even in absence of light. Anionic formulations showed no cytotoxic effect compared with free CUR and cationic CURNP and NP. The synthesized formulations improved the water solubility of CUR, showed higher antimicrobial photodynamic effect for planktonic cultures than for biofilms, and the encapsulation of CUR in anionic NP reduced the cytotoxicity of 10% DMSO used for free CUR. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 13/23165-5 - Synthesis, characterization and photodynamic effects of polymeric nanoparticle-encapsulated curcumin on in vitro biofilms of Candida albicans and on a murine model of oral candidosis
Grantee:Ewerton Garcia de Oliveira Mima
Support type: Regular Research Grants
FAPESP's process: 14/18345-7 - Photodynamic effects of polymeric nanoparticle-encapsulated curcumin on multispecies biofilm of methicillin-resistant Staphylococcus aureus and Candida albicans
Grantee:Ana Laura Mira Ortega
Support type: Scholarships in Brazil - Scientific Initiation