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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System

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Giacomini, M. C. [1] ; Scaffa, P. M. C. [2] ; Chaves, L. P. [1] ; Vidal, C. M. P. [3] ; Machado, T. N. [1] ; Honorio, H. M. [4] ; Tjaderhane, L. [5, 6, 7, 8] ; Wang, L. [1]
Total Authors: 8
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Sao Paulo - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil
[3] Univ Iowa, Coll Dent, Dept Operat Dent, Iowa City, IA 52242 - USA
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Collect Hlth, Sao Paulo - Brazil
[5] Univ Oulu, Inst Dent, Oulu - Finland
[6] Oulu Univ Hosp, Oulu - Finland
[7] Univ Turku, Inst Dent, Turku - Finland
[8] Nord Inst Dent Mat NIOM, Oslo - Norway
Total Affiliations: 8
Document type: Journal article
Source: Operative Dentistry; v. 42, n. 6, p. E188-E196, NOV-DEC 2017.
Web of Science Citations: 5

Objectives: The aim of this study was to evaluate the effect of proteolytic inhibitors on the bond strength of a universal adhesive system (etch-and-rinse mode) applied to artificial carious and eroded dentin. Methods: Ninety molars were prepared and randomly divided into three groups according to the substrate: N, no challenges; ACD, artificial carious dentin simulation and ERO, artificial erosion simulation with orange juice. All groups were redivided into three subgroups according to the dentin pretreatment: W, water; CHX, 2% digluconate chlorhexidine; and E-64 (trans-epoxysuccinyl-L-leucylamido-{[}4-guanidino] butane), 5 mu M E-64 inhibitor. They constituted a total of nine groups (n=10): N-W, N-CHX, N-E64, ACD-W, ACD-CHX, ACD-E64, ERO-W, ERO-CHX, and ERO-E64. All specimens were restored with Adper Single Bond Universal/Filtek Z250. Beams (0.64 mm(2)) were obtained and subjected to the microtensile test (mu TBS) in a universal testing machine at 0.5 mm/min. The failure mode of the interfaces was determined by optical microscopy (40X magnification). Data were statistically analyzed by three-way analysis of variance and Tukey tests (p<0.05). Results: All individual factors (p<0.0001) and the interaction between substrate and treatment (p=0.0011) and between substrate and time (p=0.0003) were statistically significant. The caries substrate contributed negatively to bond strength. Chlorhexidine reduced bond strength for normal and eroded conditions. Only the normal substrate was negatively affected by time despite the pretreatment. Conclusions: The universal bonding system appears to be a promising bonding strategy for the maintenance of bond strength to affected dentin. E-64 did not affect bonding to the dentin in contrast to the use of chlorhexidine, which, when associated with the universal system, did affect the microtensile bond strength for artificial carious dentin. (AU)

FAPESP's process: 13/12203-3 - Effect of protelolytic inhibitors on bonding to artificial carious or eroded demineralized dentin
Grantee:Marina Ciccone Giacomini Belei
Support Opportunities: Scholarships in Brazil - Master