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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

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Andre, Carolina Bosso [1] ; Rosalen, Pedro Luiz [2] ; Galvao, Livia Camara de Carualho [3, 4] ; Fronza, Bruna Mann [1] ; Boui Ambrosano, Glaucia Maria [5] ; Ferracane, Jack Liborio [6] ; Giannini, Marcelo [1]
Total Authors: 7
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[3] Dom Bosco Grad Sch, Dept Dent, Sao Luis, MA - Brazil
[4] CEUMA Univ, Sao Luis, MA - Brazil
[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Social Dent, Piracicaba, SP - Brazil
[6] Oregon Hlth & Sci Univ, Sch Dent, Dept Restorat Dent, Portland, OR 97201 - USA
Total Affiliations: 6
Document type: Journal article
Source: Dental Materials; v. 33, n. 10, p. 1084-1092, OCT 2017.
Web of Science Citations: 6

Objectives.The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods.Apigenin (1 mM) and tt-Farnesol (5 mM) were added separately and in combination to a self-etch adhesive (CS3 Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT OptiBond S).Biofilm of S.mutans was grown on adhesive-coated hydroxyapatite disks for 115 h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results.Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S.mutans biofilm.Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT.Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+ tt-Farnesol to CS3.No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Signcance.Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S.mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd.All rights reserved. (AU)

FAPESP's process: 13/22823-9 - The influence of natural products incorporation on mechanical and antimicrobial properties of adhesive systems
Grantee:Carolina Bosso André
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/17543-0 - Incorporation of natural products that modify Streptococcus mutans virulence in restorative materials
Grantee:Carolina Bosso André
Support Opportunities: Scholarships abroad - Research Internship - Doctorate