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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of soy isoflavones on the concentration of hyaluronic acid in the vagina of type 1 diabetic rats

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Carbonel, A. A. F. [1] ; Azevedo Lima, P. D. [2] ; Lim, J. J. [2] ; Teixeira Borges, F. [3] ; Rodrigues da Silva Sasso, G. [1] ; Portugal Fuchs, L. F. [4] ; Simoes, R. S. [4] ; Chada Baracat, E. [4] ; Soares, Jr., J. M. [4] ; Simoes, M. J. [1]
Total Authors: 10
[1] Univ Fed Sao Paulo, EPM UNIFESP, Dept Morphol & Genet, Paulista Sch Med, Sao Paulo - Brazil
[2] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON - Canada
[3] Univ Fed Sao Paulo, EPM UNIFESP, Dept Nephrol, Paulista Sch Med, Sao Paulo - Brazil
[4] Univ Sao Paulo, FMUSP, Dept Obstet & Gynecol, Med Fac, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CLIMACTERIC; v. 20, n. 6, p. 564-570, 2017.
Web of Science Citations: 1

Objective: To assess the effects of isoflavones and 17-estradiol on the vaginal epithelium extracellular matrix and hyaluronic acid (HA) in the diabetic rat model.Methods: Sixty adult, virgin, female rats underwent ovariectomy, then randomization into six groups of ten animals each: GI, sham ovariectomized control animals; GII, sham ovariectomized control diabetic animals; GIII, control ovariectomized rats receiving propylene glycol vehicle; GIV, control ovariectomized diabetic animals receiving propylene glycol vehicle; GV, diabetic ovariectomized animals treated with soy isoflavones (150mg/kg by gavage); GVI, ovariectomized diabetic rats treated with estrogen (17-estradiol, 10mg/kg, subcutaneously). Treatment took place over 30 consecutive days. After euthanasia, a portion of the vagina was immersed in liquid nitrogen for RT-qPCR and Western blotting. Another portion was processed for paraffin embedding. Sections were stained with hematoxylin \& eosin for histomorphometry and Picro Sirius Red for collagen quantification.Results: Vaginal epithelium histomorphometry in GIII (15.31.1 mu m) and GIV (14.5 +/- 1.8 mu m) was thinner than in GV (41.3 +/- 1.5 mu m) and GVI (74.3 +/- 1.6 mu m). There was an increase in collagen content in GV (84.1 +/- 1.2 mu m) and GVI (88.2 +/- 1.7 mu m). HA quantification was higher in GV (0.38 +/- 1.1g/mg) and GVI (0.49 +/- 1.4g/mg) when compared with GIII (0.12 +/- 1.1g/mg) and GIV (0.10 +/- 1.2g/mg), p<0.05. Conclusions: Soy isoflavones increase hyaluronic acid concentration in the vagina of diabetic ovariectomized rats. Such findings might help to attenuate the effects of vulvovaginal atrophy in women. (AU)

FAPESP's process: 14/17077-9 - Effects of isoflavones or 17beta-estradiol on cell-matrix interactions in the endometrium of diabetic rats: relevance for hormone therapy
Grantee:Adriana Aparecida Carbonel Castro Ferreira
Support type: Scholarships in Brazil - Post-Doctorate