Morphological and molecular description of Myxobolus batalhensis n. sp (Myxozoa, Myxosporea), a liver and ovary parasite of Salminus hilarii in Brazil

Author(s)

Mirandola Dias Vieira, Diego Henrique ^[1] ; Alama-Bermejo, Gema ^{[2, 3, 4]} ; Bartholomew, Jerri Lee ^[2] ; Abdallah, Vanessa Doro ^[5] ; de Azevedo, Rodney Kozlowiski ^[5]

Affiliation

^[1] Univ Estadual Paulista Julio Mesquita Filho UNESP, Dept Parasitol, Botucatu, SP - Brazil

^[2] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 - USA

^[3] Natl Univ Comahue, Ctr Appl Res & Technol Transference Marine Resour, CIMAS, CCT, CONICET, San Antonio Oeste - Argentina

^[4] Natl Univ Comahue, ESCiMar, San Antonio Oeste - Argentina

^[5] Univ Sagrado Coracao, Dept Environm Sci, Bauru, SP - Brazil

• Document type: Journal article
• Source: Parasitology Research; v. 116, n. 12, p. 3303-3313, DEC 2017.
• Web of Science Citations: 4

Abstract

Plasmodia containing myxospores belonging to the genus Myxobolus Butschli, 1882 were found in the ovaries and liver of Salminus hilarii. Despite its economic value, this fish host has no previous reports of myxozoan infections. Herein, we describe Myxobolus batalhensis n. sp. using morphological and ultrastructural data, as well as histological and SSU rDNA molecular data. The mature myxospores were elongated, measuring in average 15.2 +/- 0.8 mu m in length, 8.4 +/- 0.4 mu m in width, and 5.1 +/- 0.2 mu m in thickness. Polar capsules were elongated and measured 5.3 +/- 0.3 mu m in length and 2.8 +/- 0.3 mu m in width. Polar filaments had 6-9 coils. Histopathological analysis showed coagulation necrosis associated with cell lysis as a response of the host cell to the parasite in the ovaries. No inflammatory reaction was observed in the liver, although the presence of the plasmodia caused changes in tissue structure. The phylogenetic analysis of South American myxobolid species showed M. batalhensis n. sp. as sister species of Myxobolus aureus. This is the first report of a myxozoan species parasitizing S. hilarii and the first myxozoan species described in the Batalha river. (AU)

• FAPESP's process: 14/12862-0 - Analysis of the Mixozoaries biodiversity (Myxozoa: Myxosporea) parasites of the dominant fish species from Batalha River, middle Tietê River, SP, Brazil through morphological, histological, ultrastructural and molecular characterization
• Grantee: Rodney Kozlowiski de Azevedo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/01361-2 - Use of transmission electron microscopy and molecular techniques to study myxozoans
• Grantee: Diego Henrique Mirandola Dias Vieira
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 13/25623-0 - Application of transmission electron microscopy and histology for analysis of fish parasites mixozoários from Battle River, middle Tiête River, São Paulo, Brazil
• Grantee: Diego Henrique Mirandola Dias Vieira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/24901-2 - Phylogeny and taxonomy of myxozoans parasites of fish from inland rivers in the State of São Paulo
• Grantee: Diego Henrique Mirandola Dias Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate