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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Methionine-supplemented diet affects the expression of cardiovascular disease-related genes and increases inflammatory cytokines in mice heart and liver

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Author(s):
Aissa, Alexandre Ferro [1] ; do Amaral, Catia Lira [2] ; Venancio, Vinicius Paula [3] ; Machado, Carla da Silva [1] ; Hernandes, Livia Cristina [3] ; da Silva Santos, Patrick Wellington [3] ; Curi, Rui [4] ; Pires Bianchi, Maria de Lourdes [3] ; Greggi Antunes, Lusania Maria [3]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Goias, Campus Henrique Santillo, Anapolis, GO - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 80, n. 19-21, SI, p. 1116-1128, 2017.
Web of Science Citations: 2
Abstract

Some important environmental factors that influence the development of cardiovascular diseases (CVD) include tobacco, excess alcohol, and unhealthy diet. Methionine obtained from the diet participates in the synthesis of DNA, proteins, lipids and affects homocysteine levels, which is associated with the elevated risk for CVD development. Therefore, the aim of this study was to investigate the manner in which dietary methionine might affect cellular mechanisms underlying CVD occurrence. Swiss albino mice were fed either control (0.3% DL-methionine), methionine-supplemented (2% DL-methionine), or a methionine-deprived diet (0% DL-methionine) over a 10-week period. The parameters measured included plasma homocysteine concentrations, oxidative stress by reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, levels of inflammatory cytokines IL-1 beta, TNF-alpha, and IL-6, as well as expression of genes associated with CVD. The levels of apolipoprotein A5 (APOA5), a regulator of plasma triglycerides, were measured. The methionine-supplemented diet increased oxidative stress by lowering the GSH/GSSG ratio in heart tissues and decreased expression of the genes Apob, Ctgf, Serpinb2, Spp1, Il1b, andSell, but elevated expression of Thbs4, Tgfb2, Ccr1, andVegfa. Methionine-deprived diet reduced expression of Col3a1, Cdh5, Fabp3, Bax, andHbegf and increased expression of Sell, Ccl5, Itga2, Birc3, Msr1, Bcl2a1a, Il1r2, and Selp. Methionine-deprived diet exerted pro-inflammatory consequences as evidenced by elevated levels of cytokines IL-1 beta, TNF-alpha, and IL-6 noted in liver. Methionine-supplemented diet increased hepatic IL-6 and cardiac TNF-alpha. Both methionine supplementation and deprivation lowered hepatic levels of APOA5. In conclusion, data demonstrated that a methionine-supplemented diet modulated important biological processes associated with high risk of CVD development. (AU)

FAPESP's process: 13/02365-6 - Methylation profile, genomic instability and gene expression evaluations related with cardiovascular diseases in mice supplemented with methionine diet
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants
FAPESP's process: 10/01410-0 - Gene-diet interaction in mice supplemented with methionine in the fetal and postnatal periods: studies of genomic instability, methylation and expression of genes related to cardiovascular diseases
Grantee:Alexandre Ferro Aissa
Support type: Scholarships in Brazil - Doctorate