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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunosensor for Pancreatic Cancer Based on Electrospun Nanofibers Coated with Carbon Nanotubes or Gold Nanoparticles

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Soares, Juliana C. [1] ; Iwaki, Leonardo E. O. [1, 2] ; Soares, Andrey C. [1, 2] ; Rodrigues, Valquiria C. [1] ; Melendez, Matias E. [3] ; Fregnani, Jose Humberto T. G. [3] ; Reis, Rui M. [3, 4] ; Carvalho, Andre L. [3] ; Correa, Daniel S. [5] ; Oliveira, Jr., Osvaldo N. [1]
Total Authors: 10
[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-1356060 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mat Engn, BR-13563120 Sao Carlos, SP - Brazil
[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil
[4] Univ Minho, Life & Hlth Sci Res Inst ICVS, ICVS PT Govt Associate Lab 3Bs, P-4710057 Braga - Portugal
[5] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ACS OMEGA; v. 2, n. 10, p. 6975-6983, OCT 2017.
Web of Science Citations: 8

We report the fabrication of immunosensors based on nanostructured mats of electrospun nanofibers of polyamide 6 and poly(allylamine hydrochloride) coated either with multiwalled carbon nanotubes (MWCNTs) or gold nanoparticles (AuNPs), whose three-dimensional structure was suitable for the immobilization of anti-CA19-9 antibodies to detect the pancreatic cancer biomarker CA19-9. Using impedance spectroscopy, the sensing platform was able to detect CA19-9 with a detection limit of 1.84 and 1.57 U mL(-1) for the nanostructured architectures containing MWCNTs and AuNPs, respectively. The high sensitivity achieved can be attributed to the irreversible adsorption between antibodies and antigens, as confirmed with polarization-modulated infrared reflection absorption spectroscopy. The adsorption mechanism was typical Langmuir-Freundlich processes. The high sensitivity and selectivity of the immunosensors were also explored in tests with blood serum from patients with distinct concentrations of CA19-9, for which the impedance spectra data were processed with a multidimensional projection technique. The robustness of the immunosensors in dealing with patient samples without suffering interference from analytes present in biological fluids is promising for a simple, effective diagnosis of pancreatic cancer at early stages. (AU)

FAPESP's process: 14/16789-5 - Hybrid nanostructured materials based on conjugated polymers and metallic nanoparticles for sensor applications
Grantee:Daniel Souza Corrêa
Support type: Regular Research Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support type: Research Projects - Thematic Grants