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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Role of beta-Adrenergic Overstimulation in the Early Stages of Renal Injury

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Author(s):
de Ponte, Mariana Charleaux [1] ; Malavazzi Casare, Fernando Augusto [1] ; Costa-Pessoa, Juliana Martins [1] ; Cardoso, Vanessa Gerolde [1] ; Malnic, Gerhard [1] ; Mello-Aires, Margarida [1] ; Volpini, Rildo Aparecido [2] ; Thieme, Karina [3] ; Oliveira-Souza, Maria [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Physiol & Biophys, Lab Renal Physiol, Inst Biomed Sci, Med Sch, Sao Paulo - Brazil
[2] Univ Sao Paulo, Nephrol Dept, Lab Basic Kidney Dis LIM 12, Med Sch, Sao Paulo - Brazil
[3] Univ Sao Paulo, Med Sch, Lab Carbohydrates & Radioimmunoassays LIM 18, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Kidney & Blood Pressure Research; v. 42, n. 6, p. 1277-1289, 2017.
Web of Science Citations: 1
Abstract

Background/Aims: To assess the possible contribution of the beta-adrenergic overstimulation in early stages of renal injury, the present study evaluated, in rats, the effects of the beta-adrenoceptor agonist isoproterenol (ISO) on renal function and morphology, as well as the renal mRNA and protein expression of the NADPH oxidase isoform 4 (Nox 4) and subunit p22(phox), endoplasmic reticulum (ER) stress, pro-inflammatory, pro-apoptotic and renin-angiotensin system (RAS) components. Methods: Wistar rats received ISO (0.3 mg.kg(-1).day(-1) s.c.) or vehicle (control) for eight days. At the end of the treatment, food and water intake, urine output and body weight gain were evaluated and renal function studies were performed. Renal tissue was used for the morphological, quantitative PCR and immunohistochemical studies. Results: ISO did not change metabolic parameters or urine output. However it induced a decrease in renal blood flow and an increase in the filtration fraction. These changes were accompanied by increased cortical mRNA and protein expression for the renal oxidative stress components including Nox 4 and p22(phox); ER stress, pro-inflamatory, pro-apoptotic as well as RAS components. ISO also induced a significant increase in medullar renin protein expression. Conclusion: These findings support relevant information regarding the contribution of specific beta-adrenergic hyperactivity in early stage of renal injury, indicating the reactive oxygen species, ER stress and intrarenal RAS as important factors in this process. (c) 2017 The Author(s) Published by S. Karger AG, Basel (AU)

FAPESP's process: 14/17251-9 - The effects of chronic administration of albumin modified by advanced glycation (AGE) on renal tissue: characterization of the inflammatory, antioxidant and epigenetic profile
Grantee:Karina Thieme
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/19154-0 - Effect of angiotensin II on the cellular mechanisms involved in the podocytes injury
Grantee:Vanessa Gerolde Cardoso
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/23087-4 - Molecular and functional study of membrane transporters
Grantee:Gerhard Malnic
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/14022-0 - Effect of interaction between renin-angiotensin-aldosterone system and endothelin 1 and 3 on renal function
Grantee:Fernando Augusto Malavazzi Casare
Support type: Scholarships in Brazil - Doctorate