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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pseudomonas spp.: contamination sources in bulk tanks of dairy farms

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Author(s):
Vidal, Ana M. C. [1] ; Netto, Arlindo Saran [2] ; Vaz, Andreia C. N. [1] ; Capodifoglio, Eduardo [2] ; Goncalves, Ana C. S. [3] ; Rossi, Gabriel A. M. [3] ; Figueiredo, Annanda S. [1] ; Ruiz, Vera L. A. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, FZEA, Dept Med Vet, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, FZEA, Dept Zootecnia, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[3] Univ Estadual Paulista Unesp, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Rodovia Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 37, n. 9, p. 941-948, SEP 2017.
Web of Science Citations: 2
Abstract

This study focused on isolating Pseudomonas spp. during milking process in ten dairy farms with manual and mechanical milking systems during dry and rainy seasons, and evaluating DNA homology and patterns of distribution between isolates, in order to identify main sources of milk contamination by Pseudomonas spp. A total of 167 isolates of Pseudomonas spp. were obtained from water, milkers' hands, cows' teats, teat cups, cooling tanks and raw milk. Bacteria of Pseudomonas spp. genus were isolated from 85 and 82 sampling points in dairy farms with manual and mechanical milking system, respectively. A significant difference (p= 0.02) on Pseudomonas spp. isolation was observed among samples of surface of cows' teats before and after pre-dipping, but no significant difference (p> 0.05) was observed among milking systems or seasons. The possibility of the same Pseudomonas spp. patterns are distributed in different farms and seasons using Amplified Fragment Length Polymorphism (AFLP) technique was demonstrated. Milkers' hands, surface of cows' teats, teat cups and cooling tanks were associated with raw milk contamination with Pseudomonas spp. on farms with manual and mechanical milking system, showing that regardless of the type of milking system and season, proper hygiene procedures of equipment, utensils and workers' hands are essential to avoid contamination of the milk and, therefore, improve milk quality. (AU)

FAPESP's process: 13/04131-2 - Evaluation of quality microbial count of Pseudomonas spp. and its importance during the collection and storage of refrigerated raw milk type b during the rainy and dry
Grantee:Ana Maria Centola Vidal
Support type: Regular Research Grants