Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DEVELOPMENT AND VALIDATION OF AN HPLC-DAD ANALYTICAL METHOD TO QUANTIFY 5-METHOXYFLAVONES IN METHANOLIC EXTRACTS OF Vochysia divergens POHL CULTURED UNDER STRESS CONDITIONS

Full text
Author(s):
Pimentaa, Leticia Pereira [1] ; Kellner Filho, Luis Cladio [1] ; Soares Parpinelli, Bruna Arruda [2] ; de Siqueira, Katia Aparecida [2] ; Soares, Marcos Antonio [2] ; Andrade e Silva, Marcio Luis [1] ; Cunha, Wilson Roberto [1] ; Pauletti, Patricia Mendonca [1] ; Januario, Ana Helena [1]
Total Authors: 9
Affiliation:
[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil
[2] Univ Fed Mato Grosso, Dept Bot & Ecol, Lab Biotecnol & Ecol Microbiana, BR-78060900 Cuiaba, MT - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Química Nova; v. 40, n. 9, p. 971-975, SEP 2017.
Web of Science Citations: 1
Abstract

Vochysia divergens Pohl, known as ``Cambara{''} in Brazil, is an invasive species that is expanding throughout Pantanal in Brazil, to form mono-dominant communities. This expansion is affecting the agricultural areas that support the typical seasonal flood and drought conditions of this biome. This article describes the development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of greenhouse-grown V. divergens associated with one of two endophytic fungal species Zopfiella tetraspora (Zt) or Melanconiella elegans (Me) and later subjected to water stress. The developed method gave good validation parameters and was successfully applied to quantify the flavones 3', 5-dimethoxy luteolin-7-O-beta-glucopyranoside (1), 5-methoxy luteolin (2), and 3', 5-dimethoxy luteolin (3) in the target extracts. Inoculation of the plant with Zt decreased the concentration of flavone 1 in the extract by 2.69-fold as compared to the control. Inoculation of the plant with Zt or Me did not significantly alter the contents of flavones 2 and 3 in the extracts as compared to the control. Therefore, the aerial parts of germinated V. divergens plants inoculated with either Zt or Me responded differently in terms of the production of flavones. These results can cast light on the symbiosis between fungal microorganisms and V. divergens, which most likely influences the response of V. divergens to changes in the availability of water in Pantanal. (AU)

FAPESP's process: 13/15774-1 - Chemical Study of EtOAc fraction from Vochysia divergens and in vitro schistossomicidal potential evaluation
Grantee:Luis Claudio Kellner Filho
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/00631-5 - Bioactive compounds from medicinal and food plants of the cerrado and Pantanal and their endophytic microbiota
Grantee:Ana Helena Januário
Support type: Regular Research Grants
FAPESP's process: 13/22030-9 - HPLC/DAD/MS chemical profile of Vochysia divergens plants cultured under drought stress associated with endophytic fungi and evaluation of antioxidant and neuroprotective activities
Grantee:Letícia Pereira Pimenta
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/08057-0 - HPLC/DAD/MS chemical profile of Vochysia divergens and evaluation of their schistosomicidal, acetylcholinesterase inhibition and antioxidant potential
Grantee:Letícia Pereira Pimenta
Support type: Scholarships in Brazil - Doctorate (Direct)