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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation

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Cruz-Silva, Ilana [1] ; Nunes, Viviane Abreu [2] ; Gozzo, Andrezza Justino [3] ; Praxedes-Garcia, Priscila [1] ; Tanaka, Aparecida Sadae [1] ; Shimamoto, Kazuaki [4] ; Araujo, Mariana Silva [1]
Total Authors: 7
[1] Univ Fed Sao Paulo, Dept Biochem, Rua Tres de Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Arts Sci & Humanities, Ave Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Marine Sci, Rua Doutor Carvalho de Mendonca 144, BR-11070100 Santos, SP - Brazil
[4] Japan Hlth Care Coll, Kiyota Ku, Sinei 434-1, Sapporo, Hokkaido - Japan
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 1

Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases. (AU)

FAPESP's process: 07/55496-0 - Proteases, natural and recombinant inhibitors from Caesalpinia echinata (pau-brasil) seeds: interactions in inflammatory and neurodegenerative processes, modulated by glycosaminoglycans
Support type: Regular Research Grants
FAPESP's process: 04/11015-0 - Modulation of the kallikrein-kinin system and its receptors, influence of glycosaminoglycans, inhibitors and neurodegenerative diseases
Support type: Regular Research Grants