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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus

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Silva Evangelista, Claudia Carolina [1, 2] ; Guidelli, Giovanna Vieira [1] ; Borges, Gustavo [1] ; Araujo, Thais Fenz [1] ; Jorge de Souza, Tiago Alves [1, 2] ; da Costa Neves, Ubiraci Pereira [3] ; Tunnacliffe, Alan [4] ; Pereira, Tiago Campos [1, 2]
Total Authors: 8
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Programa Posgrad Genet, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Ribeirao Preto, SP - Brazil
[4] Univ Cambridge, Deptartment Chem Engn & Biotechnol, Cambridge - England
Total Affiliations: 4
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 40, n. 4, p. 790-802, OCT-DEC 2017.
Web of Science Citations: 1

The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holoanhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus. (AU)

FAPESP's process: 09/01520-2 - Identification of genes involved in anhydrobiosis through RNAi/viability assay following desiccation
Grantee:Tiago Campos Pereira
Support type: Scholarships in Brazil - Young Researchers