Microbicidal gentamicin-alginate hydrogels

Author(s)

Kondaveeti, Stalin ^[1] ; de Assis Bueno, Pedro Vinicius ^{[2, 1]} ; Carmona-Ribeiro, Ana Maria ; Esposito, Fernanda ^[3] ; Lincopan, Nilton ^{[4, 3]} ; Sierakowski, Maria Rita ^[5] ; Siqueira Petri, Denise Freitas ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharm, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[5] Univ Fed Parana, Chem Dept, BioPol, Curitiba, Parana - Brazil

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 186, p. 159-167, APR 15 2018.
• Web of Science Citations: 12

Abstract

Sodium alginate (Alg) reacted with antibiotic gentamicin sulfate (GS) in an aqueous-phase condition mediated by carbodiimide chemistry, in the molar ratios Alg: GS of (1:0.5), (1:1) and (1:2). The Alg-GS conjugated derivatives were characterized by elemental analysis for nitrogen content, Fourier transform infrared spectroscopy in the attenuated total reflection mode (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), thermogravimetric analyses (TGA) and water sorption measurements. XPS and FTIR-ATR analyses clearly indicated that GS molecules covalently attached to the backbone of the alginate chains by amide bond formation. The highest amount of GS bound to Alg (43.5 +/- 0.4 wt%) and the highest swelling ratio (4962 +/- 661%) were observed for the Alg-GS (1:2) sample. Bioluminescence assays with Pseudomonas aeruginosa PAO1/lecA:lux and colony forming counting of Staphylococcus aureus and Escherichia coli upon contact with all Alg-GS conjugates revealed microbicidal activity; however, Alg-GS (1:2) was the most efficient, due to the highest GS content. (AU)

• FAPESP's process: 15/25103-2 - Magnetic stimuli in biotechnological processes
• Grantee: Denise Freitas Siqueira Petri
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/14415-3 - Preparation of multicomponent polymeric magnetic materials for biotechnological applications
• Grantee: Stalin Kondaveeti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral