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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fenofibrate reverses changes induced by high-fat diet on metabolism in mice muscle and visceral adipocytes

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Author(s):
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Frias, Flavia de T. [1] ; Rocha, Karina C. E. [1] ; de Mendonca, Mariana [1] ; Murata, Gilson M. [2] ; Araujo, Hygor N. [3, 4] ; de Sousa, Luis G. O. [1] ; de Sousa, Erica [1] ; Hirabara, Sandro M. [5] ; Leite, Nayara de C. [3, 4] ; Carneiro, Everardo M. [3, 4] ; Curi, Rui [2, 5] ; Silveira, Leonardo R. [3, 4] ; Rodrigues, Alice C. [1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, Sao Paulo - Brazil
[3] Obes & Comorbid Res Ctr, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Dept Struct & Funct Biol, Inst Biol, Sao Paulo - Brazil
[5] Cruzeiro do Sul Univ, Interdisciplinary Postgrad Program Hlth Sci, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Cellular Physiology; v. 233, n. 4, p. 3515-3528, APR 2018.
Web of Science Citations: 4
Abstract

The effect of fenofibrate on the metabolism of skeletal muscle and visceral white adipose tissue of diet-induced obese (DIO) mice was investigated. C57BL/6J male mice were fed either a control or high-fat diet for 8 weeks. Fenofibrate (50mg/Kg BW, daily) was administered by oral gavage during the last two weeks of the experimental period. Insulin-stimulated glucose metabolism in soleus muscles, glucose tolerance test, insulin tolerance test, indirect calorimetry, lipolysis of visceral white adipose tissue, expression of miR-103-3p in adipose tissue, and miR-1a, miR-133a/b, miR-206, let7b-5p, miR-23b-3p, miR-29-3p, miR-143-3p in soleus muscle, genes related to glucose and fatty acid metabolism in adipose tissue and soleus muscle, and proteins (phospho-AMPK2, Pgc1, Cpt1b), intramuscular lipid staining, and activities of fatty acid oxidation enzymes in skeletal muscle were investigated. In DIO mice, fenofibrate prevented weight gain induced by HFD feeding by increasing energy expenditure; improved whole body glucose homeostasis, and in skeletal muscle, increased insulin dependent glucose uptake, miR-1a levels, reduced intramuscular lipid accumulation, and phospho-AMPK2 levels. In visceral adipose tissue of obese mice, fenofibrate decreased basal lipolysis rate and visceral adipocytes hypertrophy, and induced the expression of Glut-4, Irs1, and Cav-1 mRNA and miR-103-3p suggesting a higher insulin sensitivity of the adipocytes. The evidence is presented herein that beneficial effects of fenofibrate on body weight, glucose homeostasis, and muscle metabolism might be related to its action in adipose tissue. Moreover, fenofibrate regulates miR-1a-3p in soleus and miR-103-3p in adipose tissue, suggesting these microRNAs might contribute to fenofibrate beneficial effects on metabolism. (AU)

FAPESP's process: 11/05876-6 - Role of microRNAs in the development of insulin resistance
Grantee:Alice Cristina Rodrigues
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/24789-8 - MicroRNAs on adiponectin signalling: potential therapeutical targets of insulin resistance and insulin resistance linked diseases.
Grantee:Alice Cristina Rodrigues
Support Opportunities: Regular Research Grants