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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines

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Liberato, Tarcisio [1] ; Pessotti, Dayelle S. [1] ; Fukushima, Isabella [1] ; Kitano, Eduardo S. [2] ; Serrano, Solange M. T. [2] ; Zelanis, Andre [1]
Total Authors: 6
[1] Fed Univ Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP - Brazil
[2] Inst Butantan, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Lab Especial Toxinol Aplicada, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 174, p. 1-8, MAR 1 2018.
Web of Science Citations: 3

The imbalance of cellular homeostasis during oncogenesis together with the high heterogeneity of tumor-associated stromal cells have a marked effect on the repertoire of the proteins secreted by malignant cells (the secretome). Hence, the study of tumoral secretomes provides insights for understanding the cross-talk between cells within the tumor microenvironment as well as the key effectors for the establishment of the pre-metastatic niche in distant tumor sites. In this context, we performed a proteomic analysis of the secretomes derived from four cell lines: a paired set of fibroblasts - Hs 895. T, a cell line obtained from a lung node metastatic site from a patient who had melanoma and Hs 895.Sk, a skin fibroblast cell line (derived from the same patient); two malignant metastatic melanoma cell lines - A375, a malignant melanoma cell line from primary source and SH-4, a cell line derived from pleural effusion of a patient with metastatic melanoma. Clustering of expression profiles together with functional enrichment analysis resulted in patterns that mirrored each cell type. In addition, these patterns might be the result of cell-specific protein expression programs and reveal the emergence of trends in the co-expression of functionally related proteins in cellular melanoma models. (AU)

FAPESP's process: 14/06579-3 - System-wide analysis of N-terminal processing and protein diversity in the secretome of tumor cells
Grantee:André Zelanis Palitot Pereira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 17/07897-7 - Proteolytic processing analysis in the secretome of melanoma tumor cells
Grantee:Isabella Fukushima
Support type: Scholarships in Brazil - Scientific Initiation