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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis

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Author(s):
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Nogueira, Camila Tita [1] ; Del Cistia, Mayara Lucia [2] ; Urbaczek, Ana Carolina [3] ; Jusi, Marcia M. G. [4] ; Arenas Velasquez, Angela Maria [1] ; Machado, Rosangela Zacarias [4] ; Ferreira, Henrique [5] ; Henrique-Silva, Flavio [6] ; Langoni, Helio [7] ; da Costa, Paulo Inacio [2] ; Graminha, Marcia A. S. [2, 1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Paulista, Inst Quim, Campus Araraquara, Araraquara, SP - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Farmaceut, Campus Araraquara, Araraquara, SP - Brazil
[3] Univ Sao Paulo, Inst Quim, Campus Sao Carlos, Sao Carlos, SP - Brazil
[4] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Campus Jaboticabal, Jaboticabal, SP - Brazil
[5] Univ Estadual Paulista, Inst Biociencias, Campus Rio Claro, Rio Claro, SP - Brazil
[6] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[7] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Campus Botucatu, Botucatu, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 113, n. 3, p. 197-201, MAR 2018.
Web of Science Citations: 4
Abstract

Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant protein rLc36, expressed by Leishmania infantum, as potential antigen for more sensitive and specific diagnosis of CVL based on an immunoenzymatic assay. The concentration of 1.0 mu g/mL of rLc36 enabled differentiation of positive and negative sera and showed a sensitivity of 85% and specificity of 71% (with 95% confidence), with an accuracy of 76%. (AU)

FAPESP's process: 11/06995-9 - Searching for new antigens for canine visceral leishmaniasis serodiagnostic methods improvement: study of Lc06 and Lc36 genes.
Grantee:Camila Tita Nogueira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/15508-4 - Searching for new antigens for canine visceral Leishmaniasis serodiagnostic: study of Lc06 protein
Grantee:Mayara Lúcia Del Cistia
Support type: Scholarships in Brazil - Scientific Initiation