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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In silico characterization of tandem repeats in Trichophyton rubrum and related dermatophytes provides new insights into their role in pathogenesis

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Author(s):
Franco, Matheus Eloy [1, 2] ; Bitencourt, Tamires Aparecida [1, 3] ; Marins, Mozart [1, 4] ; Fachin, Ana Lucia [1, 4]
Total Authors: 4
Affiliation:
[1] Univ Ribeirao Preto, Unidade Biotecnol, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil
[2] Fed Inst Educ Sci & Technol South Minas Gerais IF, BR-37750000 Belo Horizonte, MG - Brazil
[3] Univ Sao Paulo, FMRP, Dept Genet, BR-049900 Sao Paulo, SP - Brazil
[4] Univ Ribeirao Preto, Curso Med, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION; JUN 11 2017.
Web of Science Citations: 0
Abstract

Trichophyton rubrum is the most common etiological agent of dermatophytoses worldwide, which is able to degrade keratinized tissues. The sequencing of the genome of different dermatophyte species has provided a large amount of data, including tandem repeats that may play a role in genetic variability and in the pathogenesis of these fungi. Tandem repeats are adjacent DNA sequences of 2-200 nucleotides in length, which exert regulatory and adaptive functions. These repetitive DNA sequences are found in different classes of fungal proteins, especially those involved in cell adhesion, a determinant factor for the establishment of fungal infection. The objective of this study was to develop a Dermatophyte Tandem Repeat Database (DTRDB) for the storage and identification of tandem repeats in T. rubrum and six other dermatophyte species. The current version of the database contains 35 577 tandem repeats detected in 16 173 coding sequences. The repeats can be searched using entry parameters such as repeat unit length (nt-nucleotide), repeat number, variability score, and repeat sequence motif. These data were used to study the relative frequency and distribution of repeats in the sequences, as well as their possible functions in dermatophytes. A search of the database revealed that these repeats occur in 22-33% of genes transcribed in dermatophytes where they could be involved in the success of adaptation to the host tissue and establishment of infection. The repeats were detected in transcripts that are mainly related to three biological processes: regulation, adhesion, and metabolism. The database developed enables users to identify and analyse tandem repeat regions in target genes related to pathogenicity and fungal-host interactions in dermatophytes and may contribute to the discovery of new targets for the development of antifungal agents. (AU)

FAPESP's process: 12/03845-9 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate human skin infection
Grantee:Ana Lucia Fachin Saltoratto
Support type: Regular Research Grants
FAPESP's process: 14/23841-3 - Study of natural compounds with antifungal activity to elucidate the action mechanism
Grantee:Ana Lucia Fachin Saltoratto
Support type: Regular Research Grants
FAPESP's process: 12/02920-7 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate infection
Grantee:Tamires Aparecida Bitencourt
Support type: Scholarships in Brazil - Doctorate