| Full text | |
| Author(s): |
Pellegrini, Vanessa O. A.
[1]
;
Bernardes, Amanda
[1]
;
Rezende, Camila A.
[2]
;
Polikarpov, Igor
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, Dept Phys & Interdisciplinary Sci, Ave Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Cellulose; v. 25, n. 3, p. 1865-1881, MAR 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
An interplay between cellulases is fundamental in biomass saccharification. Here, the synergistic action of Trichoderma harzianum Cel7A and Cel7B on two cellulosic substrates: bacterial cellulose (BC) and a much more heterogeneous filter paper (FP) was investigated by determining their saccharification yields and by analyzing both the released soluble products and the insoluble reducing ends formed in the process. Furthermore, morphological changes of the substrates were evaluated using scanning electron microscopy. Glycoside hydrolase family 7 (GH7) enzymes introduce uniform changes in BC, whereas in FP they preferentially consume thin microfibrils rather than thicker paper fibers. Thus, the size effect, which leads to a smaller surface area per unit of substrate mass for thicker fibers, seems to play a crucial role in higher enzymatic hydrolysis efficiency of BC as compared to FP. These results demonstrate that the morphology-dependent effects could be essential for the industrial breakdown of cellulose-rich plant biomass. (AU) | |
| FAPESP's process: | 12/22802-9 - Functional and Structural Studies of Carbohydrate Binding Modules of Glycosil Hydrolases |
| Grantee: | Amanda Bernardes Muniz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 10/18773-8 - Molecular cloning, expression, purification and structural characterization of endoglucanase from Trichoderma harzianum aiming the development of enzymatic blends for production of lignocellulosic ethanol |
| Grantee: | Vanessa de Oliveira Arnoldi Pellegrini |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/13602-7 - Study on strategies to allow the integral use of lignocellulosic biomass |
| Grantee: | Camila Alves de Rezende |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |