Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress

Author(s)

Adami, L. N. G. ^[1] ; Belardin, L. B. ^[1] ; Lima, B. T. ^[1] ; Jeremias, J. T. ^[1] ; Antoniassi, M. P. ^[1] ; Okada, F. K. ^[1] ; Bertolla, R. P. ^{[2, 1]}

Affiliation

^[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo - Brazil

^[2] Hosp Sao Paulo, Sao Paulo - Brazil

• Document type: Journal article
• Source: ANDROLOGIA; v. 50, n. 4 MAY 2018.
• Web of Science Citations: 2

Abstract

The aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW medium; group I: incubated with 5mmol 1-1 hydrogen peroxide; group A: incubated with 40mol 1-1 vitamin E; and group AI: incubated with both them. After incubations, sperm functional analyses were performed and included: evaluation of oxidative stress, acrosome integrity, mitochondrial activity and DNA fragmentation. Groups were compared using a Friedman test with Bonferroni post hoc (=5%). In this study, we observed that in group I there was a decrease in acrosome integrity and mitochondrial activity, and an increase in DNA fragmentation, when compared to group C. Group AI showed an increase in acrosome integrity and mitochondrial activity when compared with group I. Based on our findings, we conclude that the vitamin E supplementation had a positive effect in protecting human spermatozoon from induced oxidative stress. (AU)

• FAPESP's process: 15/02955-3 - The effect of vitamin supplements e (alpha-tocopherol) in vitro on spermatozoa submitted to oxidative stress
• Grantee: Luana Nayara Gallego Adami
• Support Opportunities: Scholarships in Brazil - Scientific Initiation