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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Can in vivo surface dental enamelmicrobiopsies be used to measure remote lead exposure?

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Author(s):
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Kaneshiro Olympio, Kelly Polido [1] ; Gonzalez Huila, Manuel Fernando [2] ; Baldini Cardoso, Cristiane de Almeida [3] ; Sacone da Silva Ferreira, Ana Paula [1] ; Ortiz, Adrielly Garcia [4] ; Toma, Henrique Eisi [2] ; Alves da Silva, Ricardo Henrique [4] ; Luz, Maciel Santos [5] ; Alves Cardoso, Maria Regina [6] ; Rodrigues Kelmer, Gislayne Aparecida [2] ; de Oliveira, Pedro Vitoriano [2] ; Henriques Bechara, Etelvino Jose [2] ; Risso Gunther, Wanda Maria [1] ; Rabelo Buzalaf, Marilia Afonso [7]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Fac Saude Publ, Dept Saude Ambiental, Av Dr Arnaldo 715, BR-01246904 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Cruzeiro Sul UNICSUL, Programa Posgrad Odontol, Area Concentracao Odontopediat, Rua Galvao Bueno 868, BR-01506000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Estomatol Saude Colet & Odontol Legal, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[5] Inst Pesquisas Tecnol Estado Sao Paulo IPT, LPM, CTMM, Av Prof Almeida Prado 532, BR-05508901 Sao Paulo, SP - Brazil
[6] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Av Dr Arnaldo 715, BR-01246904 Sao Paulo, SP - Brazil
[7] Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, Alameda Otavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Environmental Science and Pollution Research; v. 25, n. 10, SI, p. 9322-9329, APR 2018.
Web of Science Citations: 1
Abstract

Measuring lead in the surface dental enamel (SDE) using biopsies is a rapid, safe, and painless procedure. The dental enamel lead levels (DELLs) decrease from the outermost superficial layer to the inner layer of dental enamel, which becomes crucial for the biopsy depth (BD) measurement. However, whether the origin of lead found in SDE is fully endogenous is not yet established. There is also controversy about the biopsy protocol. The aims of this study were to investigate if DELLs are altered by extrinsic contamination (A) and to evaluate the real geometric figure formed by the erosion provoked by biopsy procedure and the respective BD in SDE (B). To accomplish the aim A, lead from 90 bovine incisor crowns lead was determined by graphite furnace atomic absorption spectrometer as a function of exposure time and lead concentration. Two biopsies were performed in each tooth, before and after lead exposure. Six 15-tooth groups differed by exposure time (1 or 30 min) and lead concentrations (A. 0 mg/L-placebo, B. 0.01 mg/L-standard for drinking water, or C. 0.06 mg/L-concentration found in contaminated groundwater). Phosphorus was determined by an inductively coupled plasm optical emission spectrometer to quantify the enamel removed. To compare intakes/losses of lead in SDE among the groups, values of DELL differences between before and after lead exposure were compared by ANOVA (p < 0.05). To attain the objective B, one extracted human permanent tooth was studied by confocal Raman microscopy. Lead measurements and the surface profile were determined. There was no difference in DELL among the groups (p = 0.964). The biopsy bottom surface area, analyzed by microscopy, showed an irregular area, with regions of peaks and valleys, where areas with depth ranging from 0.2 (peaks) to 1.8 mu m (valleys) (+/- 0.1 mu m) could be found. BD carried out in vivo is commonly calculated using the cylinder height formula. The real BD was shown to be very similar to already published data. In conclusion, the SDE of erupted teeth does not seem to be susceptible to environmental lead intake, being thus reliable to measure remote exposures to lead. (AU)

FAPESP's process: 01/09641-1 - Free radicals and excited species in metabolic disorders and bioluminescence
Grantee:Etelvino José Henriques Bechara
Support type: Research Projects - Thematic Grants
FAPESP's process: 06/56530-4 - Carbonilic and redox stress associated with alpha-aminoketones and endogenous beta-ketoacids: mechanisms and biomarkers.
Grantee:Etelvino José Henriques Bechara
Support type: Research Projects - Thematic Grants