New Method for Carbohydrates Determination in Sugarcane Bagasse by HPAEC-RPAD Using Glassy Carbon Electrode Modified with Carbon Nanotubes and Nickel Nanoparticles

Author(s)

de Sa, Acelino Cardoso ^{[1, 2]} ; Sedenho, Graziela Cristina ^[1] ; Paim, Leonardo Lataro ^[3] ; Stradiotto, Nelson Ramos ^{[1, 2, 4]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni 55, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Bioenergy Res Inst IPBEN, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Energy Engn, Expt Campus Rosana, Av Barrageiros 1881, Rosana, SP - Brazil

^[4] Fluminense Fed Univ UFF, Sch Ind Met Engn EEIMVR, Volta Redonda, RJ - Brazil

• Document type: Journal article
• Source: Electroanalysis; v. 30, n. 1, p. 128-136, JAN 2018.
• Web of Science Citations: 1

Abstract

Second generation ethanol can be produced from carbohydrates released from both sugarcane bagasse cell wall and sugarcane straw. The development of new method for the analysis of carbohydrates is, in this sense, seen as extremely relevant in the area of bioenergy. Based on the above considerations, the scope of this work encompasses the identification and quantification of carbohydrates composition in sugarcane bagasse without the need of sample derivatization, developing a novel analytical method using a glassy carbon electrode modified with multi-walled carbon nanotubes containing nickel oxyhydroxide nanoparticles (GCE/MWCNT/NiOOH) and applying the modified electrode as a detector in HPAEC (High Performance Anion-Exchange Chromatography) with reverse pulsed amperometric detection (RPAD) towards the determination of arabinose, galactose, glucose and xylose in hydrolyzed sugarcane bagasse. The carbohydrates concentrations determined in the hydrolyzed sugarcane bagasse were 6.1x10(-4)molL(-1), 1.0x10(-2)molL(-1) and 2.8x10(-3)molL(-1) for arabinose, glucose, and xylose respectively. Our results showed that the present method is, in essence, attractive for analysis in the course of the production process of second generation ethanol production in that it does not require sample derivatization, has rapid run time, satisfactory separation, and can be used for the detection of carbohydrates without the interference of other electroactive species. (AU)

• FAPESP's process: 12/00258-5 - Development of new methods for determination of sugars, furanic aldehydes and ironic acids from lignocellulosic materials derivatives of cane sugar
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 11/19289-5 - Determination of sugar in bagasse and straw of cane sugar by high performance liquid chromatography with electrochemical detection using electrode modified carbon nanotubes with metal oxides
• Grantee: Acelino Cardoso de Sá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/15557-3 - Determination of carbohydrates in sugarcane bagasse employing a voltammetric electronic tongue formed by nickel oxy-hydroxide/MWCNT modified electrodes
• Grantee: Acelino Cardoso de Sá
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate