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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Curcumin-mediated anti-microbial photodynamic therapy against Candida dubliniensis biofilms

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Sanita, Paula Volpato [1] ; Pavarina, Ana Claudia [1] ; Dovigo, Livia Nordi [2] ; Ribeiro, Ana Paula Dias [3] ; Andrade, Mariana Carvalho [1] ; de Oliveira Mima, Ewerton Garcia [1]
Total Authors: 6
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Social Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[3] Univ Florida, Dept Restorat Dent Sci, Coll Dent, Ctr Dr 1395, Gainesville, FL 32610 - USA
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 33, n. 4, p. 709-717, MAY 2018.
Web of Science Citations: 4

The purpose of this study was to evaluate the effectiveness of anti-microbial photodynamic therapy (aPDT) mediated by curcumin (Cur) associated with LED light against biofilms of Candida dubliniensis, and further, investigate cellular uptake and drug penetration through the biofilms under confocal laser scanning microscopy (CLSM). Four C. dubliniensis strains were tested: three clinical isolates from HIV-positive patients and one reference strain (CBS 7987). Biofilms were treated with three Cur concentrations (20.0, 30.0, and 40.0 mu M). All samples were incubated in the dark for 20 min and exposed to a 5.28 J/cm(2) of LED light fluence. Additional samples of each strain were treated either with Cur or LED light only. Control samples had neither Cur nor light. After aPDT, results were read using the XTT salt reduction method. The data were statistically analyzed by two-way ANOVA followed by Games-Howell post-hoc test (alpha = 0.05). Confocal laser scanning microscopy was used to verify both the uptake of Cur by yeast cells and its penetration through the biofilm. The results showed that aPDT promoted significant reduction on the metabolism of the biofilm-organized cells of C. dubliniensis. Further, while Cur was rapidly taken up by C. dubliniensis cells, a longer time interval was required to allow Cur penetration into biofilm cells. Based on these results, aPDT associating LED and Cur presents promising potential on fungal control of biofilms of C. dubliniensis. (AU)

FAPESP's process: 09/17975-9 - Effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the cellular damage caused to epithelial cells. In vitro and in vivo evaluation of aluminum-chloro-phthalocyanine and other derivates.
Grantee:Ana Paula Dias Ribeiro
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 10/05425-1 - Evaluation of the effectiveness of antimicrobial photodynamic therapy in the inactivation of Candida species and cellular damage caused to skin cells: in vitro and in vivo evaluation of the aluminum-chloro-phtalocyanine and other derivatives
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants