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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Induction of haem oxygenase-1 increases infection of dog macrophages by L-infantum

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Author(s):
Almeida, B. F. M. [1] ; Silva, K. L. O. [1] ; Venturin, G. L. [1] ; Chiku, V. M. [1] ; Leal, A. A. C. [1] ; Bosco, A. M. [1] ; Ciarlini, P. C. [1] ; Lima, V. M. F. [1, 2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Immunol Lab, Sch Vet Med, Aracatuba Campus, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Dept Anim Internal Med Surg & Reprod, Sch Vet Med, Aracatuba Campus, Aracatuba - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PARASITE IMMUNOLOGY; v. 39, n. 12 DEC 2017.
Web of Science Citations: 3
Abstract

We aimed to induce and inhibit HO-1, ascertaining its effect on infection rate, parasite load and the levels of superoxide, reactive oxygen species (ROS), nitric oxide (NO), TNF-alpha and IL-10 in cultured macrophages from healthy dogs infected by Leishmania infantum. Macrophages obtained from 15 healthy dogs were cultured alone or infected with L. infantum, with or without association of HO-1 inducer and inhibitor. The infection rate and the parasite load were determined by the number of infected macrophages and number of promastigotes per macrophage, respectively. HO-1 levels and gene expression, as well as IL-10 and TNF-alpha levels were also measured in these cultures. Superoxide, ROS and NO levels in macrophages were measured through flow cytometry. Induction of HO-1 increased the infection rate and parasite load, while its inhibition decreased the infection rate and IL-10 production. There was a positive correlation between HO-1 and infection rate or parasite load. Increased infection rate was associated with decreased superoxide, ROS and NO levels. Induction of HO-1 metabolism in dogs infected by L. infantum is possibly one of the mechanisms responsible for increasing the infection of macrophages, mainly through reduction in the oxidative and nitrosative metabolisms of these cells. (AU)

FAPESP's process: 13/06068-6 - The role of heme oxygenase-1 on canine visceral leishmaniasis
Grantee:Breno Fernando Martins de Almeida
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07496-1 - Role of heme oxygenase 1 in canine visceral leishmaniasis
Grantee:Valéria Marçal Felix de Lima
Support Opportunities: Regular Research Grants