Acclimatization of micropropagated of mangabeira seedlings and plant production using microorganisms growth promoter

Author(s)

Juliana Silva Rodrigues Cabral

• Document type: Doctoral Thesis
• Press: Ilha Solteira. 2016-08-31.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira
• Defense date: 2016-07-05
• Advisor: Ana Maria Rodrigues Cassiolato; Edson Luiz Souchie; Flávia Dionísio Pereira

Abstract

Mangabeira (Hancornia speciosa Gomes) stands out among the native fruit trees of the Cerrado as one of the most promising for sustainable exploitation programs. Despite the economic potential, lack of information about this culture is limiting its commercial cultivation. The sexual propagation of this species is difficult as its seeds have a short longevity, and reduced water content impairs their viability and vigor. The micropropagation technique enables the mass multiplication of plants with genetically superior features, uniform, in a reduced physical space and a short length of time. However, it eliminates the microorganisms associated with plant tissue, including mutualistcs, as mycorrhizal fungi (AMF), in addition to plant growth promoters, which can improve plant performance under stress conditions, and increase the yield. Inoculation of these microorganisms during micropropagation has been recommended to reduce the time of seedlings formation and acclimatization, and to increase tolerance to biotic and abiotic stresses, resistance to pathogens and percentage of seedling survival after transplantation. This study aimed the acclimatization of micropropagated plants with different substrates and moister chamber, inoculated with plant growth-promoting fungi (PGPF) vitro and ex vitro, associated with AMF Glomus clarum, in a greenhouse, to maximize seedlings production to be used in the mangabeira and reforestation programs. Experiments were conducted at UNESP- Universidade Estadual Paulista, Ilha Solteira Campus, and Federal Institute Goiano - Rio Verde Campus (IF Goiano - Campus Rio Verde). The plant material used in the in vitro propagation was removed from fruits of different mangabeira plants collected in Teaching, Research and Extension Farm of the UNESP Ilha Solteira Campus and Gameleira Farm, Montes Claros de Goiás-GO. All experiments were performed and conducted for 120 days. The association of plastic bag as a moist chamber with Bioplant® substrate, followed by spray PGPF and inoculation of G. clarum is the most appropriate method for acclimatization of micropropagated plants mangabeira in the greenhouse. The association of plastic bag as moist chamber with Bioplant® substrate, followed by spray PGPF and inoculation of G. clarum is the most appropriate method for acclimatization of micropropagated of mangabeira plants in the greenhouse. (AU)

• FAPESP's process: 12/14489-9 - ACCLIMATIZATION OF MICROPROPAGATED SEEDLING AND MANGABEIRA SEEDLINGS PRODUCTION USING MICROORGANISMS GROWTH PROMOTERS
• Grantee: Juliana Silva Rodrigues Cabral
• Support Opportunities: Scholarships in Brazil - Doctorate