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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diagnostic utility of DREAM gene mRNA levels in thyroid tumours

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Batista, Fernando A. [1] ; Marcello, Marjory A. [1] ; Martins, Mariana B. [1] ; Peres, Karina C. [1] ; Cardoso, Ulieme O. [1] ; Silva, Aline C. D. N. [1] ; Bufalo, Natassia E. [1] ; Soares, Fernando A. [2] ; da Silva, Marcio J. [3] ; Assumpcao, V, Ligia ; Ward, Laura S. [4]
Total Authors: 11
[1] Univ Estadual Campinas, Unicamp, FCM, Lab Genet Mol Canc Gemoca, Rua Tessalia Vieira Camargo 126, BR-13083887 Campinas, SP - Brazil
[2] Fundacao Antonio Prudente, Hosp AC Camargo, Dept Patol, Sao Paulo, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, CBMEG, Campinas, SP - Brazil
[4] Assumpcao, Ligia, V, Univ Estadual Campinas, Unicamp, FCM, Lab Genet Mol Canc Gemoca, Rua Tessalia Vieira Camargo 126, BR-13083887 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 62, n. 2, p. 205-211, MAR-APR 2018.
Web of Science Citations: 0

Objective: The transcriptional repressor DREAM is involved in thyroid-specific gene expression, thyroid enlargement and nodular development, but its clinical utility is still uncertain. In this study we aimed to investigate whether DREAM mRNA levels differ in different thyroid tumors and how this possible difference would allow the use of DREAM gene expression as molecular marker for diagnostic and/or prognosis purpose. Materials and methods: We quantified DREAM gene mRNA levels and investigated its mutational status, relating its expression and genetic changes to diagnostic and prognostic features of 200 thyroid tumors, being 101 malignant {[}99 papillary thyroid carcinomas (PTC) and 2 anaplastic thyroid carcinomas] and 99 benign thyroid lesions {[}49 goiter and 50 follicular adenomas (FA)]. Results: Levels of mRNA of DREAM gene were higher in benign (0.7909 +/- 0.6274 AU) than in malignant (0.3373 +/- 0.6274 AU) thyroid lesions (p < 0.0001). DREAM gene expression was able to identify malignancy with 66.7% sensitivity, 85.4% specificity, 84.2% positive predictive value (PPV), 68.7% negative predictive value (NPV), and 75.3% accuracy. DREAM mRNA levels were also useful distinguishing the follicular lesions FA and FVPTC with 70.2% sensitivity, 73.5% specificity, 78.5% PPV, 64.1% NPV, and 71.6% accuracy. However, DREAM gene expression was neither associated with clinical features of tumor aggressiveness, nor with recurrence or survival. Six different genetic changes in non-coding regions of DREAM gene were also found, not related to DREAM gene expression or tumor features. Conclusion: We suggest that DREAM gene expression may help diagnose thyroid nodules, identifying malignancy and characterizing follicular-patterned thyroid lesions; however, it is not useful as a prognostic marker. (AU)

FAPESP's process: 13/18683-7 - Study of transcription factors and their relation with thyroid nodules
Grantee:Ligia Vera Montali da Assumpção
Support type: Regular Research Grants