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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bioprospection of Eugenia brasiliensis, a Brazilian native fruit, as a source of anti-inflammatory and antibiofilm compounds

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Lazarini, Josy Goldoni [1] ; Orlandi Sardi, Janaina de Cassia [1] ; Franchin, Marcelo [1] ; Nani, Bruno Dias [1] ; Freires, Irlan Almeida [2] ; Infante, Juliana [3] ; Rizzato Paschoal, Jonas Augusto [4] ; de Alencar, Severino Matias [3] ; Rosalen, Pedro Luiz [1]
Total Authors: 9
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Florida, Coll Dent, Dept Oral Biol, 1395 Ctr Dr, Gainesville, FL 32610 - USA
[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ave Cafe, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BIOMEDICINE & PHARMACOTHERAPY; v. 102, p. 132-139, JUN 2018.
Web of Science Citations: 11

The anti-inflammatory and antibiofilm activities as well as toxicity and chemical profile of Eugenia brasiliensis pulp extract (EBE), were evaluated. EBE chemical profile and phenolic content were determined by LC-MS/MS. EBE was tested for its in vitro and in vivo anti-inflammatory activity, including TNF-alpha release, NF-kB activation, neutrophil migration and paw edema. The MIC/MBC and antibiofilm activities were tested against methicillin sensitive and resistant Staphylococcus aureus, Escherichia coli, Pseudomona aeruginosa, Streptococcus mutans, and Lactobacillus acidophilus. EBE acute toxicity was evaluated in Galleria mellonella and RAW 264.7 macrophage. EBE total phenolic content was 389.88 +/- 3.48 mg GAE/g with identified polyphenols. EBE decreased TNF-alpha release in vivo and in vitro, NF-kB activation, neutrophil influx into peritoneal cavity, and it showed maximal inhibition of paw edema after 2 h. MIC of EBE ranged from 62.5-500 mu g/mL while MBC values were > 500 mu g/mL, with a decrease in L. acidophilus biofilm formation. EBE showed negligible toxicity in larvae and macrophage cells. Our findings open new perspectives concerning EBE application as source of anti-inflammatory and antibiofilm molecules as a functional food, pharmaceutical lead or agribusiness commodity. (AU)

FAPESP's process: 13/13190-2 - Phenolic composition and biological potential, in vitro and in vivo, from native fruits of Brazil
Grantee:Severino Matias de Alencar
Support type: Regular Research Grants
FAPESP's process: 13/26251-0 - Prospection of anti-inflammatory activity in native fruit species from Brazil
Grantee:Josy Goldoni Lazarini
Support type: Scholarships in Brazil - Master