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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteomics as a tool to understand Leptospira physiology and virulence: Recent advances, challenges and clinical implications

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Nascimento Filho, Edson G. [1] ; Vieira, Monica L. [1] ; Teixeir, Aline F. [1] ; Santos, Jademilson C. [1] ; Fernandes, Luis G. V. [1] ; Passalia, Felipe J. [1, 2] ; Daroz, Brenda B. [1, 2] ; Rossini, Amanda [1, 2] ; Kochi, Leandro T. [1, 2] ; Cavenague, Maria F. [1, 2] ; Pimenta, Daniel C. [3] ; Nascimento, Ana L. T. O. [1]
Total Authors: 12
Affiliation:
[1] Inst Butantan, Lab Especial Desenvolvimento Vacinas, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, Sao Paulo - Brazil
[3] Inst Butantan, Lab Bioquim & Biofis, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 180, n. SI, p. 80-87, MAY 30 2018.
Web of Science Citations: 1
Abstract

Leptospirosis is a severe disease that represents a significant burden to healthcare costs in the absence of prophylactic measures. There is no effective vaccine for humans. Although Leptospira genome sequencing has broadened our current knowledge of the life cycle and evolution of these bacteria, there is still much to be understood regarding the pathophysiological features of this organism. In tropical, underdeveloped regions, it is the predominant human disease. Pathogenic bacteria of the genus Leptospira are the etiological agents of leptospirosis. This review addresses the contributions made to our knowledge of leptospirosis based on proteomic strategies. We present here the advances made on several fronts in understanding the infectious agent Leptospira, from the initial 2D-gel electrophoresis proteomics approach to more advanced mass spectrometry (MS) technologies. This review provides an update on proteomic studies of Leptospira spp. and should be constructive in further investigations of these bacteria, in our fight against leptospirosis. Impact of our work: Leptospirosis is a severe disease that represents a significant burden to healthcare costs in the absence of prophylactic measures. There is no efficient vaccine for humans. This review addresses the contribution to Leptospira knowledge based on proteomic strategies. From the initial 2D- gel electrophoreses proteomics approach to more sophisticated mass spectrometry (MS) technologies, we have compiled most recent data of authors that have performed proteomic analyses on various approaches of leptospirosis, for instance: (i) authors that have assessed the infected rat urine, searching for markers; (ii) authors that have compared the proteome profiles of bacteria grown in vivo x in vitro; (iii) authors that have searched for vaccine candidates. This is a critical review on this subject pointing out future directions on Leptospira/leptospirosis research proteomics field, envisaging rational future work to increase our knowledge of these bacteria and help fight leptospirosis. (AU)

FAPESP's process: 16/01384-5 - Evaluation of the functional role of two Leptospira interrogans proteins in the adhesion process of the pathogen to the host
Grantee:Leandro Toshio Kochi
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis
Grantee:Ana Lucia Tabet Oller Do Nascimento
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/06731-8 - Development of new strategies for genetic manipulation ín Leptospira spp.: antisense RNA ánd CRISPR/Cas9
Grantee:Luis Guilherme Virgílio Fernandes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/01102-2 - Biochemical and immunological evaluation of hypothetical surface proteins of Leptospira interrogans
Grantee:Felipe José Passalia
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/04295-3 - Interaction of two Leptospira interrogans proteins tò plasma ánd extracellular matrix components of “The host
Grantee:Maria Fernanda Cavenague Pereira
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/11541-0 - "evaluation of immunoprotective potential of CD4+ T-cell epitopes identified ín proteins of Leptospira interrogans"
Grantee:Aline Rodrigues Florêncio Teixeira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/01411-5 - Cloning, expression and characterization of HtrA proteins of Leptospira interrogans
Grantee:Brenda Daroz
Support type: Scholarships in Brazil - Master