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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects

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Vendruscolo, Cynthia do Prado [1] ; Moreira, Juliana Junqueira [1] ; Torquato Seidel, Sarah Raphaela [1] ; Fulber, Joice [1] ; Neuenschwander, Henrique Macedo [1] ; Bonagura, Giancarlo [2] ; Agreste, Fernanda Rodrigues [1] ; Arantes Baccarin, Raquel Yvonne [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Clin Med, Sao Paulo, SP - Brazil
[2] Anhembi Morumbi Univ, Dept Large Anim Clin, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 13, n. 5 MAY 29 2018.
Web of Science Citations: 1

Objective The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E-2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid. Methods The effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 mu g/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed. Results All joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P<0.05), detected by the lameness measurement system, probably because of the higher ozone concentration. All groups exhibited increased ultrasonography scores on day 14 (P<0.05). Groups A and B exhibited increased proteins concentrations on day 21 (P<0.05). There was no change in hyaluronic acid concentration or the percentage of high-molecular weight hyaluronic acid throughout the experiment. Chondroitin sulfate concentrations decreased in group B, and did not change in group A and C, indicating that neither treatment provoked extracellular matrix catabolism. Cytokine and eicosanoid concentrations were not significantly changed. Conclusions The ozonetherapy did not cause significant inflammation process or cartilage degradation, therefore, ozonetherapy is safe at both evaluated doses. (AU)

FAPESP's process: 15/04006-9 - Inflammatory and oxidant effect evaluation of the medicinal ozone in synovial joints in healthy equines
Grantee:Cynthia Do Prado Vendruscolo
Support type: Scholarships in Brazil - Master