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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preeclamptic plasma stimulates the expression of miRNAs, leading to a decrease in endothelin-1 production in endothelial cells

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Author(s):
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Caldeira-Dias, Mayara [1] ; Luizon, Marcelo R. [1] ; Deffune, Elenice [2] ; Tanus-Santos, Jose E. [3] ; Freire, Paula P. [4] ; Carvalho, Robson F. [4] ; Bettiol, Heloisa [5] ; Cardoso, Viviane C. [5] ; Barbieri, Marco Antonio [5] ; Cavalli, Ricardo C. [6] ; Sandrim, Valeria C. [1]
Total Authors: 11
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Pharmacol, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Cell Engn Lab, Blood Transfus Ctr, Botucatu Med Sch, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH; v. 12, p. 75-81, APR 2018.
Web of Science Citations: 3
Abstract

Preeclampsia is a major cause of maternal and fetal morbidity and mortality worldwide. It is a multisystem pregnancy syndrome characterized by general endothelial dysfunction caused mainly by plasma factors and debris in endothelial cells. It is widely accepted that endothelin-1 (ET-1) is involved in the pathophysiology of preeclampsia, and so it is of interest to ascertain whether the ET-1 gene (EDN1) can be targeted with tools such as miRNAs. Therefore, we investigated the relationship between the expression of miRNAs that putatively target EDN1 (and so affect ET-1 levels) in HUVECs incubated with plasma from preeclamptic women. EDN1 expression and ET-1 levels in HUVECs incubated with plasma from women with preeclampsia were similar to those in plasma from healthy pregnant women. Expression of miRNAs let-7a, -7b, and -7c, and to a lesser degree 125a and 125b, was increased in preeclampsia. Expression of miRNAs of the let-7 family was significantly negatively correlated with ET-1 levels in preeclampsia. Transfection of the preeclampsia cultures with mimic miRNA let-7 decreased ET-1 levels. Our findings show that preeclamptic plasma stimulates the expression of miRNAs in HUVECs, leading to a decrease in ET-1levels, which suggests that therapeutic miRNAs may aid in the management of preeclampsia. (AU)

FAPESP's process: 13/12919-9 - Circulating microRNA in preeclampsia
Grantee:Valeria Cristina Sandrim
Support Opportunities: Regular Research Grants