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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Expression of Lipoprotein Receptors Is Increased in the Infarcted Area After Myocardial Infarction Induced in Rats With Cardiac Dysfunction

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Author(s):
de Lima, Aline D. [1] ; Guido, Maria C. [1] ; Tavares, Elaine R. [1] ; Carvalho, Priscila O. [1] ; Marques, Alyne F. [1] ; de Melo, Marcelo D. T. [2, 3] ; Salemi, Vera M. C. [2, 3] ; Kalil-Filho, Roberto [2, 3] ; Maranhao, Raul C. [1, 4]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Med Sch Hosp, Lab Metab & Lipids, Heart Inst InCor, Lo SS, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Lo SS, Heart Failure Unit, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Lo SS, Heart Inst InCor, Clin Cardiol Div, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Lo SS, Av Dr Eneas de Carvalho Aguiar 44, Bl 2, BR-05403900 Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: LIPIDS; v. 53, n. 2, p. 177-187, FEB 2018.
Web of Science Citations: 1
Abstract

Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the status of lipoprotein receptors on the infarcted and noninfarcted areas of LV and to verify whether nano particles that mimic the lipid structure of low-density lipoprotein (LDL) and have the ability to bind to LDL receptors (LDE) are taken up more avidly by the noninfarcted LV. 13 male Wistar rats with left coronary artery ligation (myocardial infarction {[}MI]) and 12 animals with SHAM operation (SHAM) were used in this study. 6 weeks after the procedure, the quantification of low-density lipoprotein receptor (LDLR), LDL receptor-related protein 1 (LRP1), scavenger receptor-class B type I (SR-BI) lipoprotein receptors, and PCNA proliferation marker, and tissue uptake of radioactively labeled LDE were performed. Immunohistochemistry and Western blot analysis showed that LDLR, LRP1, SR-BI, and PCNA, expression in infarcted area of MI was remarkably higher than SHAM and noninfarcted subendocardial (SEN) and interstitial (INT) areas. In addition, in SEN noninfarcted area of MI, the presence of LDLR was about threefold higher than in SHAM SEN and INT noninfarcted areas. The LDE uptake of noninfarcted LV of MI group was about 30% greater than that of SHAM group. In conclusion, these findings regarding the status of lipoprotein receptors after MI induction could help to establish mechanisms on myocardial repairing. In conclusion, infarcted rats with LV dysfunction showed increased expression of lipoprotein receptors mainly in the infarcted area. (AU)

FAPESP's process: 14/03742-0 - Nanoparticles that bind to lipoprotein receptors in the treatment atherosclerosis, acute myocardial infarction, post-heart transplantation status, cancer and endometriosis
Grantee:Raul Cavalcante Maranhao
Support Opportunities: Research Projects - Thematic Grants