Protein nanoparticles are nontoxic, tuneable cell stressors

Author(s):Show less -

de Pinho Favaro, Marianna Teixeira ^{[1, 2]} ; Sanchez-Garcia, Laura ^{[2, 3, 4]} ; Sanchez-Chardi, Alejandro ^[5] ; Roldan, Monica ^[6] ; Unzueta, Ugutz ^{[2, 3, 4]} ; Serna, Naroa ^{[2, 3, 4]} ; Cano-Garrido, Olivia ^[2] ; Azzoni, Adriano Rodrigues ^[7] ; Ferrer-Miralles, Neus ^{[2, 3, 4]} ; Villaverde, Antonio ^{[2, 3, 4]} ; Vazquez, Esther ^{[2, 3, 4]}

Affiliation

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[2] Univ Autonoma Barcelona, IBB, Cerdanyola Del Valles 08193 - Spain

^[3] Univ Autonoma Barcelona, Dept Genet & Microbiol, Cerdanyola Del Valles 08193 - Spain

^[4] CIBER Bioingn Biomat & Nanomed CIBER BBN, Cerdanyola Del Valles 08193 - Spain

^[5] Univ Autonoma Barcelona, Serv Microscopia, Cerdanyola Del Valles 08193 - Spain

^[6] Hosp St Joan de Deu, IPER, Unitat Microscopia Confocal, Passeig St Joan de Deu 2, Barcelona 08950 - Spain

^[7] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Ave Prof Luciano Gualberto, Trav 3, 380, BR-05508900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Nanomedicine; v. 13, n. 3, p. 255-268, FEB 2018.
• Web of Science Citations: 3

Abstract

Aim: Nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. Results: By repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition. These materials, once exposed to cultured cells, are differentially internalized in absence of toxicity and trigger distinctive cell adaptive responses, monitored by the emission of tubular filopodia and enhanced drug sensitivity. Conclusion: The capability to rapidly modulate such cell responses by conventional protein engineering reveals protein nanoparticles as tuneable, versatile and potent cell stressors for cell-targeted conditioning. (AU)

• FAPESP's process: 15/20193-3 - Development of gene delivery vectors based on dynein light chain Rp3 and T22 peptide for CXCR4+-based cell targeting
• Grantee: Marianna Teixeira de Pinho Favaro
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate