| Full text | |
| Author(s): |
Cruz, Tais Fukuta
[1, 2]
;
Magro, Angelo Jose
[2, 3]
;
de Castro, Alessandra M. M. G.
[4, 5]
;
Pedraza-Ordonez, Francisco J.
[6]
;
Tsunemi, Miriam Harumi
[7]
;
Perahia, David
[8]
;
Araujo, Jr., Joao Pessoa
[1, 2]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Paulista Unesp, IB, Dept Microbiol & Imunol, Botucatu, SP - Brazil
[2] Univ Estadual Paulista Unesp, Inst Biotecnol IBTEC, Botucatu, SP - Brazil
[3] Univ Estadual Paulista Unesp, FCA, Dept Bioproc & Biotecnol, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil
[5] Complexo Educ Fac Metropolitanas Unidas FMU HOVET, Sao Paulo - Brazil
[6] Univ Caldas, Dept Salud Anim, Manizales, Caldas - Colombia
[7] Univ Estadual Paulista Unesp, IB, Dept Bioestat, Botucatu, SP - Brazil
[8] Univ Paris Saclay, ENS Cachan, Lab Biol & Pharmacol Appl, Cachan - France
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | VIRUS RESEARCH; v. 251, p. 22-33, JUN 2 2018. |
| Web of Science Citations: | 2 |
| Abstract | |
Porcine circovirus 2 (PCV2) is an icosahedral, non-enveloped, and single-stranded circular DNA virus that belongs to the family Circoviridae, genus Circovirus, and is responsible for a complex of different diseases defined as porcine circovirus diseases (PCVDs). These diseases - including postweaning multisystemic wasting syndrome (PMWS), enteric disease, respiratory disease, porcine dermatitis and nephropathy syndrome (PDNS), and reproductive failure - are responsible for large economic losses in the pig industry. After serial passages in swine testicle (ST) cells of a wild-type virus isolated from an animal with PMWS, we identified three PCV2b viruses with capsid protein (known as Cap protein) cumulative mutations, including two novel mutants. The mutant viruses were introduced into new ST cell cultures for reisolation and showed, in comparison to the wild-type PCV2b, remarkable viral replication efficiency ( > 10(11) DNA copies/ml) and cell death via necrosis, which were clearly related to the accretion of capsid protein mutations. The analysis of a Cap protein/capsid model showed that the mutated residues were located in solvent-accessible positions on the external PCV2b surface. Additionally, the mutated residues were found in linear epitopes and participated in pockets on the capsid surface, indicating that these residues could also be involved in antibody recognition. Taking into account the likely natural emergence of PCV2b variants, it is possible to consider that the results of this work increase knowledge of Circovirus biology and could help to prevent future serious cases of vaccine failure that could lead to heavy losses to the swine industry. (AU) | |
| FAPESP's process: | 06/59002-9 - Expressão em fagos (phage display) de anticorpos monoclonais contra o Circovirus Suino tipo 2 para aplicação em técnicas sorológicas (ELISA Enzyme Linked Immunosorbent Assay e imunoperoxidase) |
| Grantee: | João Pessoa Araújo Junior |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 06/57976-6 - Expressao em fagos ("phage display") de anticorpos monoclonais contra o circovirus suino tipo 2 para aplicacao em tecnicas sorologicas (elisa - "enzyme linked immnunosorbent assay" - e imunoperoxise). |
| Grantee: | Taís Fukuta da Cruz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/14530-1 - Structural analysis of capsid proteins and VLPs from mutant 2B swine Circovirus with distinct in vitro and in vivo pathogenic effects |
| Grantee: | Angelo José Magro |
| Support Opportunities: | Research Grants - Young Investigators Grants |