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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits

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Guedes-Monteiro, Raquel Fonseca [1] ; Ferreira-Junior, Jose Ribamar [2] ; Bleicher, Lucas [3] ; Nobrega, Francisco G. [1] ; Barrientos, Antoni [4] ; Barros, Mario H. [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo - Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG - Brazil
[4] Univ Miami, Miller Sch Med, Dept Neurol, Miami, FL 33136 - USA
Total Affiliations: 4
Document type: Journal article
Source: Cell Biology International; v. 42, n. 6, SI, p. 630-642, JUN 2018.
Web of Science Citations: 3

Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. In this work we characterize temperature sensitive mutants of the subunit bL34 present in the 54S large subunit. Although bL34 has bacterial homologs, in yeast it has a long 65 aminoacids mitochondrial N-terminal addressing sequence, here we demonstrate that it can be replaced by the mitochondrial addressing sequence of Neurospora crassa ATP9 gene. The bL34 temperature sensitive mutants present lowered translation of mitochondrial COX1 and COX3, which resulted in reduced cytochrome c oxidase activity and respiratory growth deficiency. The sedimentation properties of bL34 in sucrose gradients suggest that similarly to its bacterial homolog, bL34 is also a later participant in the process of mitoribosome biogenesis. (AU)

FAPESP's process: 13/09482-8 - Saccharomyces cerevisiae as a model for mitochondrial translation studies
Grantee:Mario Henrique de Barros
Support type: Regular Research Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 11/18892-0 - Characterization of mitochondrial gene products with unknown function in Saccharomyces Cerevisiae
Grantee:Raquel Fonseca Guedes Monteiro
Support type: Scholarships in Brazil - Doctorate