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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Central and peripheral effects of physical exercise without weight reduction in obese and lean mice

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Author(s):
de Carvalho, Francine Pereira [1] ; Moretto, Thais Ludmilla [1] ; Benfato, Izabelle Dias [1] ; Barthichoto, Marcela [2] ; Ferreira, Sandra Mara [3] ; Costa-Junior, Jose Maria [3] ; Machado de Oliveira, Camila Aparecida [4]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP - Brazil
[2] Univ Fed Sao Paulo, Programa Posgrad Alimentos Nutr & Saude, Santos, SP - Brazil
[3] Univ Estadual Campinas Unicamp, Inst Biol, Dept Biol Func & Estrutural, Campinas, SP - Brazil
[4] Univ Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BIOSCIENCE REPORTS; v. 38, n. 2 APR 27 2018.
Web of Science Citations: 5
Abstract

To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57BI/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved. (AU)

FAPESP's process: 11/05932-3 - Molecular mechanisms involved in the modulation of spontaneous physical activity and development of obesity
Grantee:Camila Aparecida Machado de Oliveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/01624-8 - Effects of different diets (low protein and high fat diet) on spontaneous physical activity and hypothalamic expression of genes related to energy homeostasis in mice
Grantee:Thais Ludmilla Moretto
Support type: Scholarships in Brazil - Master