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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ayahuasca and Kambo intoxication after alternative natural therapy for depression, confirmed by mass spectrometry

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de Morais, Damila Rodrigues [1] ; Lanaro, Rafael [2, 3] ; Barbosa, Ingrid Lopes [1] ; Santos, Jandyson Machado [1] ; Cunha, Kelly Francisco [4] ; Hernandes, Vinicius Veri [1] ; Tessaro, Elias Paulo [1] ; Gomes, Cezar Silvino [5] ; Eberlin, Marcos Nogueira [1] ; Costa, Jose Luiz [3, 6]
Total Authors: 10
[1] Univ Estadual Campinas, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Poison Control Ctr, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Fac Med Sci, Campinas, SP - Brazil
[5] Brazilian Fed Police, Natal, RN - Brazil
[6] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Forensic Toxicology; v. 36, n. 1, p. 212-221, JAN 2018.
Web of Science Citations: 0

We present a case report about an acute intoxication episode after an oral administration of Ayahuasca and dermal exposure of Kambo for treatment of depression. The clinical features observed were hallucination, agitation, tremors of extremities, oral paresthesia, skin lesions and seizures. Diazepam was administered by the emergency service and was effective in controlling hallucination, but failed to control agitation and seizures. Patient biological fluids (urine and serum) and the samples of Ayahuasca and Kambo were submitted to toxicological analysis using liquid-liquid extraction followed by liquid chromatography-tandem mass spectrometry and high-resolution electrospray ionization-mass spectrometry. The main active compounds present in Ayahuasca, N,N-dimethyltryptamine, harmine, harmaline and tetrahydroharmine were found and quantified in the different samples, confirming the use by the patient. In Kambo secretion used in the ritual, we were able to find sixteen potently active peptides: adenoregulin, bombesin, bombesinnona peptide, bradykinin -phe(8)-psi-CH2NH-arg(9)-, caerulein, deltorphin, neurokinin B, phyllomedusin, phyllocaerulein, phyllokinin, phyllolitorin, preprotachykinin B (50-79), ranatachykinin A, sauvagine, T-kinin and urechistachykinin II. The patient was discharged the day after exposure without any sequel. Clinical and toxicological analysis indicated that the symptoms presented by the patient occurred due to a joint action produced by the substances identified in both materials. To the best of our knowledge, this is the first case involving probable intoxication by simultaneous administration of Ayahuasca and Kambo. (AU)

FAPESP's process: 15/10650-8 - Stability of medicines, drugs of abuse and pesticides in dried blood spots (DBS), and its application in forensic toxicology
Grantee:José Luiz da Costa
Support type: Regular Research Grants
FAPESP's process: 16/23157-0 - Development of a protocol for new synthetic cannabinoids and NBOMe (2-(2,5-dimethoxy)-N-(2-methoxybenzyl)ethan-1-amine) derivatives) analysis in scan mode precursor ion and neutral loss by GC-MS / MS and LC-MS / MS
Grantee:Damila Rodrigues de Morais
Support type: Scholarships in Brazil - Post-Doctorate