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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

New findings from the first transcriptome of the Bothrops moojeni snake venom gland

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Author(s):
Amorim, Fernanda Gobbi [1] ; Morandi-Filho, Romualdo [2] ; Fujimura, Patricia Tieme [2] ; Ueira-Vieira, Carlos [2] ; Sampaio, Suely Vilela [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Toxinol, Ribeirao Preto, SP - Brazil
[2] Univ Fed Uberlandia, Inst Genet & Biochem, Lab Genet, Uberlandia, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Toxicon; v. 140, p. 105-117, DEC 15 2017.
Web of Science Citations: 6
Abstract

Snakebites are a serious health problem in tropical countries. In Brazil, the genus Bothrops (Viperidae family) causes most of the ophidic accidents, characterized by proteolysis and haemorrhage. Snake venoms are rich sources of toxins with great therapeutic and biotechnological potential and omits approaches is a valuable tool for identification of new bioactive components in the venom. In this study, we described the first transcriptome of the venom gland of Bothrops moojeni snake, using the next generation sequencing with the Illumina platform. We identified: (i) 20 venom components classes, among which metalloproteases were the most expressed ones, followed by serine proteases and phospholipases; and (ii) the 33 full-length amino acid sequences of toxins that have never been reported before in B. moojeni venom, such as one cysteine-rich secretory protein (Moojin), two hyaluronidases (BmooHyal-1 and BmooHyal-2), and one three-finger toxin (Bmoo-3FTx). Altogether, the transcripts identified herein represent a starting point for the analysis of structure-function relationships of toxins, which shall help develop novel biological tools and therapeutic drugs. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/20641-9 - Integration of omics approaches to profile the venom of the Bothrops moojeni snake: unreavelling new toxins and ontogenic divergences
Grantee:Fernanda Gobbi Amorim
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/26609-7 - Bothrops moojeni snake venomics: application of toxins of biotechnological interest obtained by omics techniques
Grantee:Fernanda Gobbi Amorim
Support type: Scholarships in Brazil - Post-Doctorate